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Literature summary for 3.2.1.132 extracted from

  • Price, J.S.; Storck, R.
    Production, purification, and characterization of an extracellular chitosanase from Streptomyces (1975), J. Bacteriol., 124, 1574-1585.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.132

Inhibitors

Inhibitors Comment Organism Structure
chitosan soluble chitosan, above 0.5 g/l Streptomyces sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information 0.688 mg/ml chitosan, at pH 5.5 Streptomyces sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Streptomyces sp.
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
 1 * 26000, gel filtration Streptomyces sp.
29000
-
 gel filtration Streptomyces sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
chitosan + H2O Streptomyces sp.
-
 ?
-
 ?
chitosan + H2O Streptomyces sp. No. 6
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces sp.
-
 No. 6
-
Streptomyces sp. No. 6
-
 No. 6
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information no glucosamine or other carbohydrate Streptomyces sp.

Purification (Commentary)

Purification (Comment) Organism
-
 Streptomyces sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
105
-
 diethylaminoethyl-cellulose eluate Streptomyces sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitosan + H2O
-
 Streptomyces sp. ?
-
 ?
chitosan + H2O
-
 Streptomyces sp. No. 6 ?
-
 ?
chitosan + H2O
-
 Streptomyces sp. additional information diglucosamine and triglucosamine ?
chitosan + H2O
-
 Streptomyces sp. No. 6 additional information diglucosamine and triglucosamine ?
additional information no activity with chitin (i.e. 100% acetylated chitosan) Streptomyces sp. ?
-
 ?
additional information no activity with chitin (i.e. 100% acetylated chitosan) Streptomyces sp. No. 6 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 26000, gel filtration Streptomyces sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Streptomyces sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5 6 at 25°C Streptomyces sp.