Application | Comment | Organism |
---|---|---|
synthesis | application in enzymatic production of xylooligosaccharides from xylan. The high level of the thermostable alpha-glucuronidase from Thermotoga maritima, combined purification by a simple heat treament, has a considerable potential in the production of xylooligosaccharide, especially xylobiose | Thermotoga maritima |
Cloned (Comment) | Organism |
---|---|
expression of the gene in Escherichia coli is very low, even in an expression plasmid construct with optimized transcription and translation start sequences. Rare codons for arginine (AGA and AGG) and isoleucine (AUA) affect the AguA from Thermothoga maritima in Escherichia coli. x03In order to raise gene expression to meet industrial requirements, the rare codons in the N-terminal region of the AguA from Thermotoga maritima are replaced, and the aguA gene is overexpressed using the heat-inducible vector pHsh. The application of the recombinant enzyme in the production of xylobioses from xylan is also described | Thermotoga maritima |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | P96105 | - |
- |
Thermotoga maritima DSM 3109 | P96105 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-O-methyl-D-glucuronoxylan + H2O | the enzyme is able to remove 4-O-methylglucuronic acid groups from 4-O-methyl-D-glucuronoxylan | Thermotoga maritima | 4-O-methyl-alpha-D-glucuronic acid + ? | - |
? | |
4-O-methyl-D-glucuronoxylan + H2O | the enzyme is able to remove 4-O-methylglucuronic acid groups from 4-O-methyl-D-glucuronoxylan | Thermotoga maritima DSM 3109 | 4-O-methyl-alpha-D-glucuronic acid + ? | - |
? | |
birchwood xylan + H2O | the enzyme is able to remove 4-O-methylglucuronic acid groups from birchwood xylan | Thermotoga maritima | 4-O-methyl-alpha-D-glucuronic acid + ? | - |
? | |
birchwood xylan + H2O | the enzyme is able to remove 4-O-methylglucuronic acid groups from birchwood xylan | Thermotoga maritima DSM 3109 | 4-O-methyl-alpha-D-glucuronic acid + ? | - |
? | |
oat spelt xylan + H2O | the enzyme is able to remove 4-O-methylglucuronic acid groups from birchwood xylan | Thermotoga maritima | 4-O-methyl-alpha-D-glucuronic acid + ? | - |
? | |
oat spelt xylan + H2O | the enzyme is able to remove 4-O-methylglucuronic acid groups from birchwood xylan | Thermotoga maritima DSM 3109 | 4-O-methyl-alpha-D-glucuronic acid + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AguA | - |
Thermotoga maritima |
xylanolytic alpha-glucuronidase | - |
Thermotoga maritima |
General Information | Comment | Organism |
---|---|---|
metabolism | one of the rate-limiting enzymes for the degradation of xylan | Thermotoga maritima |