Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Geobacillus stearothermophilus |
Crystallization (Comment) | Organism |
---|---|
two crystal forms, form T1 is obtained by the vapour-diffusion method using polyethylene glycol as precipitant and 2-propanol as organic additive, tetragonal space group P41212 or P43212 with a and b: 76.1 A and c: 331.2 A, form M1 at slightly lower pH and lower concentration of 2-propanol, monoclinic space group P21 with a: 65.8 A, b: 127.4 A, c: 96.6 A and beta: 97.9° | Geobacillus stearothermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
78480 | - |
alpha2, 2 * 78480, deduced from nucleotide sequence | Geobacillus stearothermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
xylan + H2O | Geobacillus stearothermophilus | - |
4-O-methyl-alpha-D-glucuronic acid + ? | - |
? | |
xylan + H2O | Geobacillus stearothermophilus T-6 | - |
4-O-methyl-alpha-D-glucuronic acid + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
T-6 | - |
Geobacillus stearothermophilus T-6 | - |
T-6 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
xylan + H2O | - |
Geobacillus stearothermophilus | 4-O-methyl-alpha-D-glucuronic acid + ? | - |
? | |
xylan + H2O | liberates 4-O-methyl-D-glucuronic acid from the main chain of the hardwood xylan | Geobacillus stearothermophilus | 4-O-methyl-alpha-D-glucuronic acid + ? | - |
? | |
xylan + H2O | - |
Geobacillus stearothermophilus T-6 | 4-O-methyl-alpha-D-glucuronic acid + ? | - |
? | |
xylan + H2O | liberates 4-O-methyl-D-glucuronic acid from the main chain of the hardwood xylan | Geobacillus stearothermophilus T-6 | 4-O-methyl-alpha-D-glucuronic acid + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | alpha2, 2 * 78480, deduced from nucleotide sequence | Geobacillus stearothermophilus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
5.42 |