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Literature summary for 3.2.1.131 extracted from

  • Teplitsky, A.; Shulami, S.; Moryles, S.; Zaide, G.; Shoham, Y.; Shoham, G.
    Crystallization and preliminary X-ray analysis of alpha-D-glucuronidase from Bacillus stearothermophilus T-6 (1999), Acta Crystallogr. Sect. D, 55, 869-872.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Geobacillus stearothermophilus

Crystallization (Commentary)

Crystallization (Comment) Organism
two crystal forms, form T1 is obtained by the vapour-diffusion method using polyethylene glycol as precipitant and 2-propanol as organic additive, tetragonal space group P41212 or P43212 with a and b: 76.1 A and c: 331.2 A, form M1 at slightly lower pH and lower concentration of 2-propanol, monoclinic space group P21 with a: 65.8 A, b: 127.4 A, c: 96.6 A and beta: 97.9° Geobacillus stearothermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
78480
-
alpha2, 2 * 78480, deduced from nucleotide sequence Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
xylan + H2O Geobacillus stearothermophilus
-
4-O-methyl-alpha-D-glucuronic acid + ?
-
?
xylan + H2O Geobacillus stearothermophilus T-6
-
4-O-methyl-alpha-D-glucuronic acid + ?
-
?

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
T-6
-
Geobacillus stearothermophilus T-6
-
T-6
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
xylan + H2O
-
Geobacillus stearothermophilus 4-O-methyl-alpha-D-glucuronic acid + ?
-
?
xylan + H2O liberates 4-O-methyl-D-glucuronic acid from the main chain of the hardwood xylan Geobacillus stearothermophilus 4-O-methyl-alpha-D-glucuronic acid + ?
-
?
xylan + H2O
-
Geobacillus stearothermophilus T-6 4-O-methyl-alpha-D-glucuronic acid + ?
-
?
xylan + H2O liberates 4-O-methyl-D-glucuronic acid from the main chain of the hardwood xylan Geobacillus stearothermophilus T-6 4-O-methyl-alpha-D-glucuronic acid + ?
-
?

Subunits

Subunits Comment Organism
dimer alpha2, 2 * 78480, deduced from nucleotide sequence Geobacillus stearothermophilus

pI Value

Organism Comment pI Value Maximum pI Value
Geobacillus stearothermophilus
-
-
5.42