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Literature summary for 3.2.1.131 extracted from

  • Nurizzo, D.; Nagy, T.; Gilbert, H.J.; Davies, G.J.
    The structural basis for catalysis and specificity of the Pseudomonas cellulosa alpha-glucuronidase, GlcA67A (2002), Structure, 10, 547-556.
    View publication on PubMed
Search for more literature for 3.2.1.131

Cloned(Commentary)

Cloned (Comment) Organism
expression as a His10-thioredoxin fusion protein in Escherichia coli Cellvibrio japonicus

Crystallization (Commentary)

Crystallization (Comment) Organism
vapor phase diffusion using the hanging drop method, 250 mM MgCl2 and 20% v/v ethylene glycol with 15% w/v polyethylene glycol 3350 as prcipitant at 20°C, triclinic space group P1 with a: 69.9 A, b: 74.7 A, c: 87.6 A, alpha: 115.2°, beta: 93.1° and gamma: 109.3° Cellvibrio japonicus

Protein Variants

Protein Variants Comment Organism
D365A much less active than wild type Cellvibrio japonicus
D365C much less active than wild type Cellvibrio japonicus
E292A much less active than wild type Cellvibrio japonicus
E292C much less active than wild type Cellvibrio japonicus
E393A much less active than wild type Cellvibrio japonicus
E393C less active than wild type Cellvibrio japonicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.28
-
 aldotriouronic acid 37°C, pH 6.5 Cellvibrio japonicus
0.36
-
 aldotetraouronic acid 37°C, pH 6.5 Cellvibrio japonicus
0.42
-
 aldopentaouronic acid 37°C, pH 6.5 Cellvibrio japonicus
0.53
-
 aldobiouronic acid 37°C, pH 6.5 Cellvibrio japonicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
77000
-
 alpha2, 2 * 77000, SDS-PAGE, crystal structure Cellvibrio japonicus
150000
-
 gel filtration Cellvibrio japonicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
xylan + H2O Cellvibrio japonicus
-
 4-O-methyl-alpha-D-glucuronic acid + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Cellvibrio japonicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Cellvibrio japonicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
aldobiouronic acid + H2O
-
 Cellvibrio japonicus 4-O-methyl-alpha-D-glucuronic acid + ?
-
 ?
aldopentaouronic acid + H2O
-
 Cellvibrio japonicus 4-O-methyl-D-glucuronic acid + Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xyl
-
 ?
aldotetraouronic acid + H2O
-
 Cellvibrio japonicus 4-O-methyl-D-glucuronic acid + Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xyl
-
 ?
aldotriouronic acid + H2O
-
 Cellvibrio japonicus 4-O-methyl-alpha-D-glucuronic acid + Xylbeta(1-4)Xylbeta(1-4)Xyl
-
 ?
xylan + H2O
-
 Cellvibrio japonicus 4-O-methyl-alpha-D-glucuronic acid + ?
-
 ?
xylan + H2O liberates 4-O-methyl-D-glucuronic acid from the main chain of the hardwood xylan Cellvibrio japonicus 4-O-methyl-alpha-D-glucuronic acid + ?
-
 ?
xylan + H2O
-
 Cellvibrio japonicus xylobiose + ?
-
 ?

Subunits

Subunits Comment Organism
dimer alpha2, 2 * 77000, SDS-PAGE, crystal structure Cellvibrio japonicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
69.2
-
 aldobiouronic acid 37°C, pH 6.5 Cellvibrio japonicus
96
-
 aldotriouronic acid 37°C, pH 6.5 Cellvibrio japonicus
103
-
 aldotetraouronic acid 37°C, pH 6.5 Cellvibrio japonicus
132
-
 aldopentaouronic acid 37°C, pH 6.5 Cellvibrio japonicus