Literature summary for 3.2.1.131 extracted from

Zaide, G.; Shallom, D.; Shulami, S.; Zolotnitsky, G.; Golan, G.; Baasov, T.; Shoham, G.; Shoham, Y.
Biochemical characterization and identification of catalytic residues in alpha-glucuronidase from Bacillus stearothermophilus T-6 (2001), Eur. J. Biochem., 268, 3006-3016.

Search for more literature for 3.2.1.131

Cloned(Commentary)

Cloned (Comment)	Organism
overexpression in Escherichia coli	Geobacillus stearothermophilus

Protein Variants

Protein Variants	Comment	Organism
AguADELTAC	lacks the last 81 amino acids, heat labile, 0.1% of the specific activity of the wild-type enzyme at 35°C	Geobacillus stearothermophilus
AguADELTAN	lacks the first 126 amino acids, 1% of the specific activity of the wild-type enzyme at 55°C	Geobacillus stearothermophilus
AguADELTANC	lacks the first 126 and last 81 amino acids, is insoluble	Geobacillus stearothermophilus
D274A	no change in activity	Geobacillus stearothermophilus
D364A	great decrease in activity	Geobacillus stearothermophilus
E158N	decrease in activity	Geobacillus stearothermophilus
E285N	decrease in activity	Geobacillus stearothermophilus
E386Q	decrease in activity	Geobacillus stearothermophilus
E392C	great decrease in activity	Geobacillus stearothermophilus
E510A	decrease in activity	Geobacillus stearothermophilus

Inhibitors

Inhibitors	Comment	Organism	Structure
Ag+	-	Geobacillus stearothermophilus
Cu2+	-	Geobacillus stearothermophilus
Hg2+	-	Geobacillus stearothermophilus
K+	-	Geobacillus stearothermophilus
Ni2+	-	Geobacillus stearothermophilus
Zn2+	-	Geobacillus stearothermophilus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.2	-	aldotetraouronic acid	55°C, pH 5,5	Geobacillus stearothermophilus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
78480	-	alpha2, 2 * 78480, deduced from nucleotide sequence	Geobacillus stearothermophilus
150000	-	gel filtration	Geobacillus stearothermophilus

Organism

Organism	UniProt	Comment	Textmining
Geobacillus stearothermophilus	-	T-6	-
Geobacillus stearothermophilus T-6	-	T-6	-

Purification (Commentary)

Purification (Comment)	Organism
-	Geobacillus stearothermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
42	-	after gel filtration	Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
aldotetraouronic acid + H2O	-	Geobacillus stearothermophilus	4-O-methyl-D-glucuronic acid + Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xyl	-	?
aldotetraouronic acid + H2O	-	Geobacillus stearothermophilus T-6	4-O-methyl-D-glucuronic acid + Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xyl	-	?

Subunits

Subunits	Comment	Organism
dimer	alpha2, 2 * 78480, deduced from nucleotide sequence	Geobacillus stearothermophilus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
65	-	-	Geobacillus stearothermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
70	-	fully active after 20 min	Geobacillus stearothermophilus
73.4	-	denaturation temperature	Geobacillus stearothermophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
54.9	-	aldotetraouronic acid	55°C, pH 5,5	Geobacillus stearothermophilus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5.5	6	-	Geobacillus stearothermophilus

pH Range

pH Minimum	pH Maximum	Comment	Organism
4.5	7.5	at pH 4.5 and 7.5 retains 40% of enzyme activity	Geobacillus stearothermophilus

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)