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Literature summary for 3.2.1.130 extracted from

  • Tulsiani, D.R.P.; Coleman, V.D.; Touster, O.
    Asparagine-linked glycoprotein biosynthesis in rat brain: identification of glucosidase I, glucosidase II, and and endomannosidase (glucosyl mannosidase) (1990), Arch. Biochem. Biophys., 277, 114-121.
    View publication on PubMed
Search for more literature for 3.2.1.130

Activating Compound

Activating Compound Comment Organism Structure
Triton X-100 requirement, membrane-bound enzyme Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Rattus norvegicus 16020
-
microsome intrinsic microsomal membrane component Rattus norvegicus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
380000
-
 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GlcMan9GlcNAc + H2O Rattus norvegicus biosynthesis of N-linked glycoproteins Man8GlcNAc + glucosyl alpha1,3-mannose
-
 ?
GlcMan9GlcNAc + H2O Rattus norvegicus Wistar biosynthesis of N-linked glycoproteins Man8GlcNAc + glucosyl alpha1,3-mannose
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 Wistar
-
Rattus norvegicus Wistar
-
 Wistar
-

Purification (Commentary)

Purification (Comment) Organism
partial Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Glc2Man9GlcNAc + H2O at 14% the rate of GlcMan9GlcNAc hydrolysis Rattus norvegicus ?
-
 ?
Glc3Man9GlcNAc2 + H2O at 11% the rate of GlcMan9GlcNAc hydrolysis Rattus norvegicus ?
-
 ?
GlcMan7GlcNAc + H2O at 71% the rate of GlcMan9GlcNAc hydrolysis Rattus norvegicus ?
-
 ?
GlcMan7GlcNAc + H2O at 71% the rate of GlcMan9GlcNAc hydrolysis Rattus norvegicus Wistar ?
-
 ?
GlcMan8GlcNAc + H2O at 89% the rate of GlcMan9GlcNAc hydrolysis Rattus norvegicus ?
-
 ?
GlcMan8GlcNAc + H2O at 89% the rate of GlcMan9GlcNAc hydrolysis Rattus norvegicus Wistar ?
-
 ?
GlcMan9GlcNAc + H2O best substrate Rattus norvegicus Man8GlcNAc + glucosyl alpha1,3-mannose
-
 ?
GlcMan9GlcNAc + H2O biosynthesis of N-linked glycoproteins Rattus norvegicus Man8GlcNAc + glucosyl alpha1,3-mannose
-
 ?
GlcMan9GlcNAc + H2O best substrate Rattus norvegicus Wistar Man8GlcNAc + glucosyl alpha1,3-mannose
-
 ?
GlcMan9GlcNAc + H2O biosynthesis of N-linked glycoproteins Rattus norvegicus Wistar Man8GlcNAc + glucosyl alpha1,3-mannose
-
 ?
additional information little or no activity with di- or triglucosylated oligosaccharide as substrate Rattus norvegicus ?
-
 ?
additional information little or no activity with di- or triglucosylated oligosaccharide as substrate Rattus norvegicus Wistar ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2 6.8
-
 Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
5.5 7.5 about half-maximal activity at pH 5.8 and 7.7 Rattus norvegicus