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Literature summary for 3.2.1.130 extracted from

  • Lubas, W.A.; Spiro, R.G.
    Golgi endo-alpha-D-mannosidase from rat liver, a novel N-linked carbohydrate unit processing enzyme (1987), J. Biol. Chem., 262, 3775-3781.
    View publication on PubMed
Search for more literature for 3.2.1.130

Inhibitors

Inhibitors Comment Organism Structure
additional information not impaired by 1-deoxynojirimycin, no inhibition by EDTA Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum rough endoplasmic reticulum Rattus norvegicus 5783
-
Golgi membrane
-
 Rattus norvegicus 139
-
membrane
-
 Rattus norvegicus 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GlcMan9GlcNAc + H2O Rattus norvegicus
-
 Man8GlcNAc + glucosyl alpha1,3-mannose
-
 ?
GlcMan9GlcNAc + H2O Rattus norvegicus Cd
-
 Man8GlcNAc + glucosyl alpha1,3-mannose
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 CD-strain
-
Rattus norvegicus Cd
-
 CD-strain
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Storage Stability

Storage Stability Organism
-70°C, isolated membranes, storage for 40 days with several freeze-thawings results in about a 30% loss in activity Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GlcMan9GlcNAc + H2O
-
 Rattus norvegicus Man8GlcNAc + glucosyl alpha1,3-mannose
-
 ?
GlcMan9GlcNAc + H2O
-
 Rattus norvegicus Cd Man8GlcNAc + glucosyl alpha1,3-mannose
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
4 9 about half-maximal activity at pH 6 and 8.5 Rattus norvegicus