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Literature summary for 3.2.1.129 extracted from

  • Berski, S.; van Bergeijk, J.; Schwarzer, D.; Stark, Y.; Kasper, C.; Scheper, T.; Grothe, C.; Gerardy-Schahn, R.; Kirschning, A.; Draeger, G.
    Synthesis and biological evaluation of a polysialic acid-based hydrogel as enzymatically degradable scaffold material for tissue engineering (2008), Biomacromolecules, 9, 2353-2359.
    View publication on PubMed
Search for more literature for 3.2.1.129

Application

Application Comment Organism
biotechnology degradation of non-toxic modified polysialic acid hydrogel scaffold in neuro-regenerative tissue engineering (4.26 microgram enzyme + 39 mg hydrogel), in phosphate buffered saline (400 microl, pH 7.4), at 37°C, degradation speed 2-11 days depending on cross-linker amount (0.6, 0.8, 2 equivalents diepoxyoctane, no activity with 3 equivalents diepoxyoctane), hydrogel was coated with collagen I, poly-L-lysine/collagen I, or diluted matrigel for neurite formation in PC12 cells Escherichia phage K1F
degradation degradation of non-toxic modified polysialic acid hydrogel scaffold in neuro-regenerative tissue engineering (4.26 microgram enzyme + 39 mg hydrogel), in phosphate buffered saline (400 microl, pH 7.4), at 37°C, degradation speed 2-11 days depending on cross-linker amount (0.6, 0.8, 2 equivalents diepoxyoctane, no activity with 3 equivalents diepoxyoctane), hydrogel was coated with collagen I, poly-L-lysine/collagen I, or diluted matrigel for neurite formation in PC12 cells Escherichia phage K1F
medicine degradation of non-toxic modified polysialic acid hydrogel scaffold in neuro-regenerative tissue engineering (4.26 microgram enzyme + 39 mg hydrogel), in phosphate buffered saline (400 microl, pH 7.4), at 37°C, degradation speed 2-11 days depending on cross-linker amount (0.6, 0.8, 2 equivalents diepoxyoctane, no activity with 3 equivalents diepoxyoctane), hydrogel was coated with collagen I, poly-L-lysine/collagen I, or diluted matrigel for neurite formation in PC12 cells Escherichia phage K1F

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
alpha-2,8-linked polysialic acid + H2O Escherichia phage K1F
-
 oligomers of polysialic acid
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia phage K1F
-
 enzyme lacking the N-terminal head-binding domain
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-2,8-linked polysialic acid + H2O
-
 Escherichia phage K1F oligomers of polysialic acid
-
 ?
alpha-2,8-linked polysialic acid cross-linked by diepoxyoctane + H2O polysialic acid hydrogel consisting of 20% (w/v) polysialic acid and a minimum of 0.6 equivalents diepoxyoctane, pH 7.4, 37°C Escherichia phage K1F oligomers of polysialic acid
-
 ?

Synonyms

Synonyms Comment Organism
endo-N-acetylneuraminidase
-
 Escherichia phage K1F
endoNF proteolyitcally processed endosialidase lacking the N-terminal head-binding domain Escherichia phage K1F
endosialidase
-
 Escherichia phage K1F