Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli as fusion protein | Vectrevirus K1E |
Protein Variants | Comment | Organism |
---|---|---|
D138A | lower Km than wild-type enzyme | Vectrevirus K1E |
D154A | lower Km than wild-type enzyme | Vectrevirus K1E |
DELTA DI | insoluble enzyme | Vectrevirus K1E |
DELTA DII | insoluble enzyme | Vectrevirus K1E |
DELTA P-loop | higher Km than wild-type enzyme | Vectrevirus K1E |
G153A | less turnover than wild-type enzyme | Vectrevirus K1E |
Trunc N45 | lower Km than wild-type enzyme | Vectrevirus K1E |
W159A | insoluble enzyme | Vectrevirus K1E |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | 17.7 | polysialic acid | 37°C, pH 6.5, G153A | Vectrevirus K1E | |
3.7 | - |
polysialic acid | 37°C, pH 6.5, Trunc N45 | Vectrevirus K1E | |
4.2 | 17.7 | polysialic acid | 37°C, pH 6.5, wild-type | Vectrevirus K1E | |
6.4 | - |
polysialic acid | 37°C, pH 6.5, D154A | Vectrevirus K1E | |
8.3 | - |
polysialic acid | 37°C, pH 6.5, D138A | Vectrevirus K1E | |
27.1 | - |
polysialic acid | 37°C, pH 6.5, DELTA P-loop | Vectrevirus K1E |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
76000 | - |
x * 76000, SDS-PAGE | Vectrevirus K1E |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
poly(sialic) acids or oligo(sialic) acids containing alpha-2,8-linked N-acetylneuraminic acid + H2O | Vectrevirus K1E | - |
fragments of poly(sialic) acid or oligo(sialic) acids | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vectrevirus K1E | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Vectrevirus K1E |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
poly(sialic) acids or oligo(sialic) acids containing alpha-2,8-linked N-acetylneuraminic acid + H2O | - |
Vectrevirus K1E | fragments of poly(sialic) acid or oligo(sialic) acids | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 76000, SDS-PAGE | Vectrevirus K1E |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | 1 | polysialic acid | 37°C, pH 6.5, G153A | Vectrevirus K1E | |
0.2 | - |
polysialic acid | 37°C, pH 6.5, D138A | Vectrevirus K1E | |
0.4 | 1.5 | polysialic acid | 37°C, pH 6.5, wild-type | Vectrevirus K1E | |
0.5 | - |
polysialic acid | 37°C, pH 6.5, Trunc N45 | Vectrevirus K1E | |
1.2 | - |
polysialic acid | 37°C, pH 6.5, D154A | Vectrevirus K1E | |
1.4 | - |
polysialic acid | 37°C, pH 6.5, DELTA P-loop | Vectrevirus K1E |