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Literature summary for 3.2.1.129 extracted from

  • Leggate, D.R.; Bryant, J.M.; Redpath, M.B.; Head, D.; Taylor, P.W.; Luzio, J.P.
    Expression, mutagenesis and kinetic analysis of recombinant K1E endosialidase to define the site of proteolytic processing and requirements for catalysis (2002), Mol. Microbiol., 44, 749-760.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli as fusion protein Vectrevirus K1E

Protein Variants

Protein Variants Comment Organism
D138A lower Km than wild-type enzyme Vectrevirus K1E
D154A lower Km than wild-type enzyme Vectrevirus K1E
DELTA DI insoluble enzyme Vectrevirus K1E
DELTA DII insoluble enzyme Vectrevirus K1E
DELTA P-loop higher Km than wild-type enzyme Vectrevirus K1E
G153A less turnover than wild-type enzyme Vectrevirus K1E
Trunc N45 lower Km than wild-type enzyme Vectrevirus K1E
W159A insoluble enzyme Vectrevirus K1E

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1 17.7 polysialic acid 37°C, pH 6.5, G153A Vectrevirus K1E
3.7
-
polysialic acid 37°C, pH 6.5, Trunc N45 Vectrevirus K1E
4.2 17.7 polysialic acid 37°C, pH 6.5, wild-type Vectrevirus K1E
6.4
-
polysialic acid 37°C, pH 6.5, D154A Vectrevirus K1E
8.3
-
polysialic acid 37°C, pH 6.5, D138A Vectrevirus K1E
27.1
-
polysialic acid 37°C, pH 6.5, DELTA P-loop Vectrevirus K1E

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
76000
-
x * 76000, SDS-PAGE Vectrevirus K1E

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
poly(sialic) acids or oligo(sialic) acids containing alpha-2,8-linked N-acetylneuraminic acid + H2O Vectrevirus K1E
-
fragments of poly(sialic) acid or oligo(sialic) acids
-
?

Organism

Organism UniProt Comment Textmining
Vectrevirus K1E
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Vectrevirus K1E

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
poly(sialic) acids or oligo(sialic) acids containing alpha-2,8-linked N-acetylneuraminic acid + H2O
-
Vectrevirus K1E fragments of poly(sialic) acid or oligo(sialic) acids
-
?

Subunits

Subunits Comment Organism
? x * 76000, SDS-PAGE Vectrevirus K1E

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.01 1 polysialic acid 37°C, pH 6.5, G153A Vectrevirus K1E
0.2
-
polysialic acid 37°C, pH 6.5, D138A Vectrevirus K1E
0.4 1.5 polysialic acid 37°C, pH 6.5, wild-type Vectrevirus K1E
0.5
-
polysialic acid 37°C, pH 6.5, Trunc N45 Vectrevirus K1E
1.2
-
polysialic acid 37°C, pH 6.5, D154A Vectrevirus K1E
1.4
-
polysialic acid 37°C, pH 6.5, DELTA P-loop Vectrevirus K1E