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Literature summary for 3.2.1.127 extracted from

  • Yazawa, S.; Madiyalakan, R.; Chawda, R.P.; Matta, K.L.
    alpha-L-Fucosidase from Aspergillus niger: demonstration of a novel alpha-L-(1--6)-fucosidase acting on glycopeptides (1986), Biochem. Biophys. Res. Commun., 136, 563-569.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00000277
-
alpha-L-fucosyl asialo-agalacto-fetuin
-
Aspergillus niger

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
180000
-
gel filtration Aspergillus niger

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
immunoglobulin G glycopeptide + H2O Aspergillus niger
-
fragments of immunoglobuline G glycopeptide
-
?

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial, 156fold Aspergillus niger

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.000000606
-
-
Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-1,6-fucosyl asialo-agalacto-fetuin + H2O
-
Aspergillus niger alpha-L-fucose + asialo-agalacto-fetuin
-
?
alpha-L-fucosyl asialo-agalacto-fetuin + H2O
-
Aspergillus niger alpha-L-fucose + asialo-agalacto-fetuin
-
?
immunoglobulin G glycopeptide + H2O
-
Aspergillus niger fragments of immunoglobuline G glycopeptide
-
?
immunoglobulin G glycopeptide + H2O
-
Aspergillus niger fragments of immunoglobuline G glycopeptide alpha-1,6-fucosyl linkage is cleaved ?
additional information alpha-1,2-, alpha-1,3- or alpha-1,4-alpha-L-fucosides are not cleaved, alpha-L-fucosyl asialo-fetuin is no substrate Aspergillus niger ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
Aspergillus niger