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Literature summary for 3.2.1.126 extracted from

  • Escamilla-Trevino, L.L.; Chen, W.; Card, M.L.; Shih, M.C.; Cheng, C.L.; Poulton, J.E.
    Arabidopsis thaliana beta-glucosidases BGLU45 and BGLU46 hydrolyse monolignol glucosides (2006), Phytochemistry, 67, 1651-1660.
    View publication on PubMed
Search for more literature for 3.2.1.126

Cloned(Commentary)

Cloned (Comment) Organism
cDNAs encoding BGLU45 and BGLU46 expressed in Pichia pastoris Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.2
-
 p-coumaryl beta-D-glucoside BGLU46 Arabidopsis thaliana
5.1
-
 syringin BGLU45 Arabidopsis thaliana
7
-
 coniferin BGLU45 Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
 ecotype Columbia
-

Purification (Commentary)

Purification (Comment) Organism
to apparent homogeneity by ammonium sulfate fractionation and hydrophobic interaction chromatography, BGLU45 8.8fold and BGLU46 13.7fold purified Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
additional information BGLU45 and BGLU46 absent from rosette leaves or flowers Arabidopsis thaliana
-
root BGLU46 Arabidopsis thaliana
-
silique BGLU45 Arabidopsis thaliana
-
stem BGLU45 and BGLU46 display increasing levels of expression from apex to base, matching the known increase in lignification Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1
-
 BGLU45, crude extract Arabidopsis thaliana
35.9
-
 BGLU45, purified enzyme Arabidopsis thaliana
43.8
-
 BGLU46, crude extract Arabidopsis thaliana
599
-
 BGLU46, purified enzyme Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-amygdalin
-
 Arabidopsis thaliana ?
-
 ?
4-methylumbelliferyl-beta-D-glucoside 9% activity with BGLU46 Arabidopsis thaliana 4-methylumbelliferol + beta-D-glucose
-
 ?
arbutin 6% activity with BGLU46 Arabidopsis thaliana benzene-1,4-diol + beta-D-glucose
-
 ?
coniferin 87% activity with BGLU45 and 8% activity with BGLU46 Arabidopsis thaliana beta-D-glucose + coniferyl aldehyde
-
 ?
additional information no activity with sinigrin, methyl-beta-D-glucoside, D(+)cellobiose, beta-gentiobiose, D(+)maltose, sucrose, p-nitrophenyl-1-thio-beta-D-glucopyranoside, p-nitrophenyl-alpha-D-glucopyranoside, p-nitrophenyl-N-acetyl-beta-D-glucosaminide, p-nitrophenyl-beta-D-mannopyranoside, p-nitrophenyl-beta-D-xylopyranoside, p-nitrophenyl-beta-L-fucopyranoside and p-nitrophenyl-beta-L-arabinopyranoside Arabidopsis thaliana ?
-
 ?
o-nitrophenyl-beta-D-glucopyranoside most rapidly hydrolysed by BGLU45 and BGLU46 with relative rates of 619% and 449%, respectively, of the rate of p-nitrophenyl-beta-D-glucoside degradation Arabidopsis thaliana o-nitrophenol + beta-D-glucose
-
 ?
p-coumaryl beta-D-glucoside 6.9% activity with BGLU45 and 71% activity with BGLU46 Arabidopsis thaliana p-coumaric acid + beta-D-glucose
-
 ?
p-nitrophenyl-beta-D-galactopyranoside 0.4% activity with BGLU45 and 21% activity with BGLU46 Arabidopsis thaliana p-nitrophenol + beta-D-galactose
-
 ?
p-nitrophenyl-beta-D-glucopyranoside 100% activity with BGLU45 and BGLU46 Arabidopsis thaliana p-nitrophenol + beta-D-glucose
-
 ?
phenyl-beta-D-glucoside 62% activity with BGLU46 Arabidopsis thaliana phenol + beta-D-glucose
-
 ?
salicin 100% activity with BGLU46 Arabidopsis thaliana beta-D-glucose + salicyl alcohol
-
 ?
syringin + H2O 100% activity with BGLU45 and 6% activity with BGLU46 Arabidopsis thaliana D-glucose + sinapyl alcohol
-
 ?

Synonyms

Synonyms Comment Organism
beta-glucosidase
-
 Arabidopsis thaliana
BGLU45
-
 Arabidopsis thaliana
BGLU46
-
 Arabidopsis thaliana