Literature summary for 3.2.1.125 extracted from

Xia, L.; Lin, H.; Staniek, A.; Panjikar, S.; Ruppert, M.; Hilgers, P.; Williardt, J.; Rajendran, C.; Wang, M.; Warzecha, H.; J�ger, V.; St�ckigt, J.
Ligand structures of synthetic deoxa-pyranosylamines with raucaffricine and strictosidine glucosidases provide structural insights into their binding and inhibitory behaviours (2015), J. Enzyme Inhib. Med. Chem., 30, 472-478 .

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Cloned(Commentary)

Cloned (Comment)	Organism
heterologously expressed in Escherichia coli	Rauvolfia serpentina

Crystallization (Commentary)

Crystallization (Comment)	Organism
enzyme in complex with the (1R,2S,3S,4R,5R)-4-(cyclohexylmethylamino)-5-(hydroxymethyl)cyclopentane-1,2,3-triol	Rauvolfia serpentina

Inhibitors

Inhibitors	Comment	Organism	Structure
N-(bromobenzyl)-beta-D-gluco-1,5-deoxa-pyranosylamine	the inhibitor anchors exclusively in the catalytic active site by competition with appropriate enzyme substrates	Rauvolfia serpentina
N-(cyclohexyl)-beta-D-gluco-1,5-deoxa-pyranosylamine	the inhibitor anchors exclusively in the catalytic active site by competition with appropriate enzyme substrates	Rauvolfia serpentina
N-(cyclohexylmethyl)-beta-D-gluco-1,5-deoxa-pyranosylamine	the inhibitor anchors exclusively in the catalytic active site by competition with appropriate enzyme substrates	Rauvolfia serpentina

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
raucaffricine + H2O	Rauvolfia serpentina	-	D-glucose + vomilenine	-	?

Organism

Organism	UniProt	Comment	Textmining
Rauvolfia serpentina	Q9SPP9	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
raucaffricine + H2O	-	Rauvolfia serpentina	D-glucose + vomilenine	-	?

Synonyms

Synonyms	Comment	Organism
raucaffricine glucosidase	-	Rauvolfia serpentina

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Rauvolfia serpentina

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5	-	-	Rauvolfia serpentina

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.000066	-	N-(bromobenzyl)-beta-D-gluco-1,5-deoxa-pyranosylamine	30°C, pH 5.0	Rauvolfia serpentina
0.00022	-	N-(cyclohexylmethyl)-beta-D-gluco-1,5-deoxa-pyranosylamine	30°C, pH 5.0	Rauvolfia serpentina
0.628	-	N-(cyclohexyl)-beta-D-gluco-1,5-deoxa-pyranosylamine	30°C, pH 5.0	Rauvolfia serpentina

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Release 2023.1 (January 2023)