Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.125 extracted from

  • Stoeckigt, J.; Panjikar, S.; Ruppert, M.; Barleben, L.; Ma, X.; Loris, E.; Hill, M.
    The molecular architecture of major enzymes from ajmaline biosynthetic pathway (2007), Phytochem. Rev., 6, 15-34.
No PubMed abstract available
Search for more literature for 3.2.1.125

Cloned(Commentary)

Cloned (Comment) Organism
for heterologous expression, RG cDNA cloned into the pSE280 vector. For optimum expression, RG cDNA cloned into the pQE-2 vector and expressed in Escherichia coli strain M15 Rauvolfia serpentina

Crystallization (Commentary)

Crystallization (Comment) Organism
by the hanging-drop vapour diffusion technique at 20°C. Crystals reach a maximum dimension of about 0.2 * 0.15 * 0.05 mm, belong to space group I222 and diffract to 2.30 A resolution with unit-cell parameters of a = 102.8 A, b = 127.3 A and c = 215.8 A Rauvolfia serpentina

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3
-
 Raucaffricine
-
 Rauvolfia serpentina

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60930
-
 sequence analysis Rauvolfia serpentina
61000
-
 isolated from cell suspension Rauvolfia serpentina

Organism

Organism UniProt Comment Textmining
Rauvolfia serpentina
-
-
-

Purification (Commentary)

Purification (Comment) Organism
1200fold, by Ni-NTA column Rauvolfia serpentina

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-dihydroraucaffricine + H2O
-
 Rauvolfia serpentina 2beta(R)-1,2-dihydrovomilenine + D-glucose
-
 ?
raucaffricine + H2O best substrate Rauvolfia serpentina D-glucose + vomilenine
-
 ?
strictosidine + H2O
-
 Rauvolfia serpentina ?
-
 ?

Subunits

Subunits Comment Organism
dimer crystallography Rauvolfia serpentina
monomer in solution Rauvolfia serpentina

Synonyms

Synonyms Comment Organism
raucaffricine glucosidase
-
 Rauvolfia serpentina