Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dimethylformamide | the activity is enhanced by addition of 5% (v/v) dimethylformamide | Rhodococcus equi | |
Dimethylsulfoxide | the activity is enhanced by addition of 10% (v/v) dimethylsulfoxide | Rhodococcus equi | |
tetrahydrofuran | the activity is enhanced by addition of 5% (v/v) tetrahydrofuran | Rhodococcus equi | |
Triton X-100 | the activity is enhanced by addition of 0.1% (v/v) Triton X-100 | Rhodococcus equi |
Cloned (Comment) | Organism |
---|---|
expressed in Rhodococcus erythropolis JCM3201 cells | Rhodococcus equi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.23 | - |
ganglioside Gb3Cer | isoform EHCase II, pH 5.5 and 37°C | Rhodococcus equi | |
0.4 | - |
ganglioside GM1a | isoform EHCase I, pH 5.5 and 37°C | Rhodococcus equi | |
0.43 | - |
ganglioside GM1a | isoform EHCase II, pH 5.5 and 37°C | Rhodococcus equi | |
0.46 | - |
ganglioside Gb3Cer | isoform EHCase I, pH 5.5 and 37°C | Rhodococcus equi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
x * 55000, isoform EGCase I, SDS-PAGE | Rhodococcus equi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
oligoglycosylglucosyl-(1<->1)-ceramide + H2O | Rhodococcus equi | - |
ceramide + oligoglycosylglucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus equi | - |
formerly Rhodococcus sp. strain M-750 | - |
Purification (Comment) | Organism |
---|---|
Ni Sepharose 6 column chromatography | Rhodococcus equi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-L-fucosyl-(1->2)-ganglioside GM1a + H2O | isoform EGCase I exhibits 100% activity with this substrate | Rhodococcus equi | ? | - |
? | |
Forssman glycolipid + H2O | isoform EGCase I exhibits 25.2% activity with this substrate | Rhodococcus equi | ? | - |
? | |
ganglioside Gb3Cer + H2O | isoform EGCase I exhibits 100% activity with this substrate | Rhodococcus equi | ? | - |
? | |
ganglioside Gb4Cer + H2O | isoform EGCase I exhibits 33.9% activity with this substrate | Rhodococcus equi | ? | - |
? | |
ganglioside GD1a + H2O | isoform EGCase I exhibits 100% activity with this substrate | Rhodococcus equi | ? | - |
? | |
ganglioside GD1b + H2O | isoform EGCase I exhibits 100% activity with this substrate | Rhodococcus equi | ? | - |
? | |
ganglioside GD3 + H2O | isoform EGCase I exhibits 100% activity with this substrate | Rhodococcus equi | ? | - |
? | |
ganglioside GM1a + H2O | isoform EGCase I exhibits 100% activity with this substrate | Rhodococcus equi | ? | - |
? | |
ganglioside GM3 + H2O | isoform EGCase I exhibits 100% activity with this substrate | Rhodococcus equi | ? | - |
? | |
glucosylceramide + H2O | isoform EGCase I exhibits 6.7% activity with this substrate | Rhodococcus equi | ? | - |
? | |
lactosylceramide + H2O | isoform EGCase I exhibits 29.9% activity with this substrate | Rhodococcus equi | ? | - |
? | |
additional information | almost no hydrolysis is observed for isoform EGCase II with alpha-L-fucosyl-(1->2)-ganglioside GM1a, ganglioside Gb3Cer, and ganglioside Gb4Cer. Isoform EGCase I shows no activity with ganglioside GM4, trigalactosylceramide, sulfatide, and sphingomyelin | Rhodococcus equi | ? | - |
? | |
additional information | for transglycosylation activity, isoform EGCase I prefers alkanols with a relatively long carbon chain such as pentanol and hexanol as an acceptor substrate | Rhodococcus equi | ? | - |
? | |
oligoglycosylglucosyl-(1<->1)-ceramide + H2O | - |
Rhodococcus equi | ceramide + oligoglycosylglucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 55000, isoform EGCase I, SDS-PAGE | Rhodococcus equi |
Synonyms | Comment | Organism |
---|---|---|
EGCase I | isoform | Rhodococcus equi |
EGCase II | isoform | Rhodococcus equi |
endoglycoceramidase | - |
Rhodococcus equi |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.069 | - |
ganglioside Gb3Cer | isoform EHCase II, pH 5.5 and 37°C | Rhodococcus equi | |
5.7 | - |
ganglioside GM1a | isoform EHCase II, pH 5.5 and 37°C | Rhodococcus equi | |
85 | - |
ganglioside Gb3Cer | isoform EHCase I, pH 5.5 and 37°C | Rhodococcus equi | |
120 | - |
ganglioside GM1a | isoform EHCase I, pH 5.5 and 37°C | Rhodococcus equi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | 6 | isoform EGCase I | Rhodococcus equi |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
ganglioside Gb3Cer | isoform EHCase II, pH 5.5 and 37°C | Rhodococcus equi | |
13 | - |
ganglioside GM1a | isoform EHCase II, pH 5.5 and 37°C | Rhodococcus equi | |
190 | - |
ganglioside Gb3Cer | isoform EHCase I, pH 5.5 and 37°C | Rhodococcus equi | |
320 | - |
ganglioside GM1a | isoform EHCase I, pH 5.5 and 37°C | Rhodococcus equi |