BRENDA - Enzyme Database
show all sequences of 3.2.1.123

Preparation and characterization of EGCase I, applicable to the comprehensive analysis of GSLs, using a rhodococcal expression system

Ishibashi, Y.; Kobayashi, U.; Hijikata, A.; Sakaguchi, K.; Goda, H.M.; Tamura, T.; Okino, N.; Ito, M.; J. Lipid Res. 53, 2242-2251 (2012)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
dimethylformamide
the activity is enhanced by addition of 5% (v/v) dimethylformamide
Rhodococcus hoagii
Dimethylsulfoxide
the activity is enhanced by addition of 10% (v/v) dimethylsulfoxide
Rhodococcus hoagii
tetrahydrofuran
the activity is enhanced by addition of 5% (v/v) tetrahydrofuran
Rhodococcus hoagii
Triton X-100
the activity is enhanced by addition of 0.1% (v/v) Triton X-100
Rhodococcus hoagii
Cloned(Commentary)
Cloned (Commentary)
Organism
expressed in Rhodococcus erythropolis JCM3201 cells
Rhodococcus hoagii
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.23
-
ganglioside Gb3Cer
isoform EHCase II, pH 5.5 and 37°C
Rhodococcus hoagii
0.4
-
ganglioside GM1a
isoform EHCase I, pH 5.5 and 37°C
Rhodococcus hoagii
0.43
-
ganglioside GM1a
isoform EHCase II, pH 5.5 and 37°C
Rhodococcus hoagii
0.46
-
ganglioside Gb3Cer
isoform EHCase I, pH 5.5 and 37°C
Rhodococcus hoagii
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
55000
-
x * 55000, isoform EGCase I, SDS-PAGE
Rhodococcus hoagii
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
oligoglycosylglucosyl-(1<->1)-ceramide + H2O
Rhodococcus hoagii
-
ceramide + oligoglycosylglucose
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Rhodococcus hoagii
-
formerly Rhodococcus sp. strain M-750
-
Purification (Commentary)
Purification (Commentary)
Organism
Ni Sepharose 6 column chromatography
Rhodococcus hoagii
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
alpha-L-fucosyl-(1->2)-ganglioside GM1a + H2O
isoform EGCase I exhibits 100% activity with this substrate
732295
Rhodococcus hoagii
?
-
-
-
?
ganglioside Forssman + H2O
isoform EGCase I exhibits 25.2% activity with this substrate
732295
Rhodococcus hoagii
?
-
-
-
?
ganglioside Gb3Cer + H2O
isoform EGCase I exhibits 100% activity with this substrate
732295
Rhodococcus hoagii
?
-
-
-
?
ganglioside Gb4Cer + H2O
isoform EGCase I exhibits 33.9% activity with this substrate
732295
Rhodococcus hoagii
?
-
-
-
?
ganglioside GD1a + H2O
isoform EGCase I exhibits 100% activity with this substrate
732295
Rhodococcus hoagii
?
-
-
-
?
ganglioside GD1b + H2O
isoform EGCase I exhibits 100% activity with this substrate
732295
Rhodococcus hoagii
?
-
-
-
?
ganglioside GD3 + H2O
isoform EGCase I exhibits 100% activity with this substrate
732295
Rhodococcus hoagii
?
-
-
-
?
ganglioside GM1a + H2O
isoform EGCase I exhibits 100% activity with this substrate
732295
Rhodococcus hoagii
?
-
-
-
?
ganglioside GM3 + H2O
isoform EGCase I exhibits 100% activity with this substrate
732295
Rhodococcus hoagii
?
-
-
-
?
glucosylceramide + H2O
isoform EGCase I exhibits 6.7% activity with this substrate
732295
Rhodococcus hoagii
?
-
-
-
?
lactosylceramide + H2O
isoform EGCase I exhibits 29.9% activity with this substrate
732295
Rhodococcus hoagii
?
-
-
-
?
additional information
almost no hydrolysis is observed for isoform EGCase II with alpha-L-fucosyl-(1->2)-ganglioside GM1a, ganglioside Gb3Cer, and ganglioside Gb4Cer. Isoform EGCase I shows no activity with ganglioside GM4, trigalactosylceramide, sulfatide, and sphingomyelin
732295
Rhodococcus hoagii
?
-
-
-
-
additional information
for transglycosylation activity, isoform EGCase I prefers alkanols with a relatively long carbon chain such as pentanol and hexanol as an acceptor substrate
732295
Rhodococcus hoagii
?
-
-
-
-
oligoglycosylglucosyl-(1<->1)-ceramide + H2O
-
732295
Rhodococcus hoagii
ceramide + oligoglycosylglucose
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 55000, isoform EGCase I, SDS-PAGE
Rhodococcus hoagii
Synonyms
Synonyms
Commentary
Organism
EGCase I
isoform
Rhodococcus hoagii
EGCase II
isoform
Rhodococcus hoagii
endoglycoceramidase
-
Rhodococcus hoagii
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.069
-
ganglioside Gb3Cer
isoform EHCase II, pH 5.5 and 37°C
Rhodococcus hoagii
5.7
-
ganglioside GM1a
isoform EHCase II, pH 5.5 and 37°C
Rhodococcus hoagii
85
-
ganglioside Gb3Cer
isoform EHCase I, pH 5.5 and 37°C
Rhodococcus hoagii
120
-
ganglioside GM1a
isoform EHCase I, pH 5.5 and 37°C
Rhodococcus hoagii
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.5
6
isoform EGCase I
Rhodococcus hoagii
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
dimethylformamide
the activity is enhanced by addition of 5% (v/v) dimethylformamide
Rhodococcus hoagii
Dimethylsulfoxide
the activity is enhanced by addition of 10% (v/v) dimethylsulfoxide
Rhodococcus hoagii
tetrahydrofuran
the activity is enhanced by addition of 5% (v/v) tetrahydrofuran
Rhodococcus hoagii
Triton X-100
the activity is enhanced by addition of 0.1% (v/v) Triton X-100
Rhodococcus hoagii
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Rhodococcus erythropolis JCM3201 cells
Rhodococcus hoagii
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.23
-
ganglioside Gb3Cer
isoform EHCase II, pH 5.5 and 37°C
Rhodococcus hoagii
0.4
-
ganglioside GM1a
isoform EHCase I, pH 5.5 and 37°C
Rhodococcus hoagii
0.43
-
ganglioside GM1a
isoform EHCase II, pH 5.5 and 37°C
Rhodococcus hoagii
0.46
-
ganglioside Gb3Cer
isoform EHCase I, pH 5.5 and 37°C
Rhodococcus hoagii
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
55000
-
x * 55000, isoform EGCase I, SDS-PAGE
Rhodococcus hoagii
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
oligoglycosylglucosyl-(1<->1)-ceramide + H2O
Rhodococcus hoagii
-
ceramide + oligoglycosylglucose
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
Ni Sepharose 6 column chromatography
Rhodococcus hoagii
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
alpha-L-fucosyl-(1->2)-ganglioside GM1a + H2O
isoform EGCase I exhibits 100% activity with this substrate
732295
Rhodococcus hoagii
?
-
-
-
?
ganglioside Forssman + H2O
isoform EGCase I exhibits 25.2% activity with this substrate
732295
Rhodococcus hoagii
?
-
-
-
?
ganglioside Gb3Cer + H2O
isoform EGCase I exhibits 100% activity with this substrate
732295
Rhodococcus hoagii
?
-
-
-
?
ganglioside Gb4Cer + H2O
isoform EGCase I exhibits 33.9% activity with this substrate
732295
Rhodococcus hoagii
?
-
-
-
?
ganglioside GD1a + H2O
isoform EGCase I exhibits 100% activity with this substrate
732295
Rhodococcus hoagii
?
-
-
-
?
ganglioside GD1b + H2O
isoform EGCase I exhibits 100% activity with this substrate
732295
Rhodococcus hoagii
?
-
-
-
?
ganglioside GD3 + H2O
isoform EGCase I exhibits 100% activity with this substrate
732295
Rhodococcus hoagii
?
-
-
-
?
ganglioside GM1a + H2O
isoform EGCase I exhibits 100% activity with this substrate
732295
Rhodococcus hoagii
?
-
-
-
?
ganglioside GM3 + H2O
isoform EGCase I exhibits 100% activity with this substrate
732295
Rhodococcus hoagii
?
-
-
-
?
glucosylceramide + H2O
isoform EGCase I exhibits 6.7% activity with this substrate
732295
Rhodococcus hoagii
?
-
-
-
?
lactosylceramide + H2O
isoform EGCase I exhibits 29.9% activity with this substrate
732295
Rhodococcus hoagii
?
-
-
-
?
additional information
almost no hydrolysis is observed for isoform EGCase II with alpha-L-fucosyl-(1->2)-ganglioside GM1a, ganglioside Gb3Cer, and ganglioside Gb4Cer. Isoform EGCase I shows no activity with ganglioside GM4, trigalactosylceramide, sulfatide, and sphingomyelin
732295
Rhodococcus hoagii
?
-
-
-
-
additional information
for transglycosylation activity, isoform EGCase I prefers alkanols with a relatively long carbon chain such as pentanol and hexanol as an acceptor substrate
732295
Rhodococcus hoagii
?
-
-
-
-
oligoglycosylglucosyl-(1<->1)-ceramide + H2O
-
732295
Rhodococcus hoagii
ceramide + oligoglycosylglucose
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 55000, isoform EGCase I, SDS-PAGE
Rhodococcus hoagii
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.069
-
ganglioside Gb3Cer
isoform EHCase II, pH 5.5 and 37°C
Rhodococcus hoagii
5.7
-
ganglioside GM1a
isoform EHCase II, pH 5.5 and 37°C
Rhodococcus hoagii
85
-
ganglioside Gb3Cer
isoform EHCase I, pH 5.5 and 37°C
Rhodococcus hoagii
120
-
ganglioside GM1a
isoform EHCase I, pH 5.5 and 37°C
Rhodococcus hoagii
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.5
6
isoform EGCase I
Rhodococcus hoagii
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.3
-
ganglioside Gb3Cer
isoform EHCase II, pH 5.5 and 37°C
Rhodococcus hoagii
13
-
ganglioside GM1a
isoform EHCase II, pH 5.5 and 37°C
Rhodococcus hoagii
190
-
ganglioside Gb3Cer
isoform EHCase I, pH 5.5 and 37°C
Rhodococcus hoagii
320
-
ganglioside GM1a
isoform EHCase I, pH 5.5 and 37°C
Rhodococcus hoagii
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.3
-
ganglioside Gb3Cer
isoform EHCase II, pH 5.5 and 37°C
Rhodococcus hoagii
13
-
ganglioside GM1a
isoform EHCase II, pH 5.5 and 37°C
Rhodococcus hoagii
190
-
ganglioside Gb3Cer
isoform EHCase I, pH 5.5 and 37°C
Rhodococcus hoagii
320
-
ganglioside GM1a
isoform EHCase I, pH 5.5 and 37°C
Rhodococcus hoagii
Other publictions for EC 3.2.1.123
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
750248
Shen
Mass spectrometry-based ident ...
Rhodococcus sp. M-777
Carbohydr. Res.
476
53-59
2019
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
751102
Han
Structural insights into the ...
Rhodococcus hoagii 103S, Rhodococcus hoagii, Rhodococcus sp. M-777
J. Biol. Chem.
292
4789-4800
2017
-
-
1
1
12
-
-
2
-
-
-
-
-
5
-
-
-
-
-
-
8
-
15
-
4
-
-
-
2
-
-
-
-
-
-
-
-
-
1
-
1
12
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
8
-
15
-
-
-
-
2
-
-
-
-
-
-
-
-
2
2
749565
Albrecht
Comprehensive profiling of gl ...
Rhodococcus triatomea, Rhodococcus triatomea BKS 15-14
Anal. Chem.
88
4795-4802
2016
-
2
1
-
-
-
-
-
-
-
-
-
-
4
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
749905
Ben Bdira
Hydrophobic interactions cont ...
Rhodococcus sp.
Biochemistry
55
4823-4835
2016
-
-
-
-
-
1
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
732178
Watanabe
Sterylglucoside catabolism in ...
Cryptococcus neoformans, Cryptococcus neoformans H99
J. Biol. Chem.
290
1005-1019
2015
-
-
1
-
-
-
1
5
-
-
-
-
-
5
1
-
1
-
-
-
-
-
13
-
6
1
1
-
5
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
5
-
-
-
-
-
1
-
1
-
-
-
-
13
-
1
1
-
5
1
1
-
-
-
-
-
-
5
5
732295
Ishibashi
Preparation and characterizati ...
Rhodococcus hoagii
J. Lipid Res.
53
2242-2251
2012
4
-
1
-
-
-
-
4
-
-
1
1
-
4
-
-
1
-
-
-
-
-
14
1
3
-
-
-
4
1
-
-
-
-
-
-
4
-
1
-
-
-
-
-
-
-
4
-
-
1
1
-
-
-
1
-
-
-
-
14
1
-
-
-
4
1
-
-
-
-
-
-
-
4
4
714723
Rich
Glycosphingolipid synthesis em ...
Rhodococcus sp.
Chem. Commun. (Camb. )
47
10806-10808
2011
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
715127
Karlsson
Differentiation of glycosphing ...
Rhodococcus sp.
Glycobiology
20
1103-1116
2010
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
7
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
698135
Li
Preparation of homogenous olig ...
Rhodococcus sp., Rhodococcus sp. M-750
Glycoconj. J.
26
929-933
2009
1
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
15
-
3
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
15
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
699823
Misonou
Comprehensive clinico-glycomic ...
Rhodococcus sp.
J. Proteome Res.
8
2990-3005
2009
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
703891
Xu
A novel fucosyl glycosphingoli ...
Rhodococcus sp.
Glycobiology
19
1446-1451
2009
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
703903
Ishibashi
Transglycosylation-based fluor ...
Rhodococcus hoagii
Glycobiology
19
797-807
2009
-
-
1
-
-
-
-
-
-
-
-
-
-
5
-
-
1
-
-
-
-
-
10
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
10
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
695545
Hancock
High-throughput screening of c ...
Rhodococcus sp.
Anal. Biochem.
382
48-54
2008
-
-
1
-
7
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
677532
Korekane
Novel fucogangliosides found i ...
Rhodococcus sp.
Anal. Biochem.
364
37-50
2007
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
677557
Caines
The structural basis of glycos ...
Rhodococcus sp.
Angew. Chem. Int. Ed. Engl.
46
4474-4476
2007
-
-
1
1
-
-
3
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
3
-
-
-
-
1
-
1
-
-
-
3
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
680549
Xu
Fucosyl-GM1a, an endoglycocera ...
Sus scrofa
J. Biochem.
141
1-7
2007
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
1
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
680753
Ishibashi
A novel endoglycoceramidase hy ...
Rhodococcus hoagii
J. Biol. Chem.
282
11386-11396
2007
-
-
1
-
4
-
6
1
-
-
2
-
-
3
-
-
1
-
-
-
-
-
6
1
5
-
-
-
1
1
-
-
-
-
1
-
-
-
1
-
-
4
-
-
6
-
1
-
-
2
-
-
-
-
1
-
-
-
-
6
1
-
-
-
1
1
-
-
1
-
-
-
-
-
-
680770
Caines
Structural and mechanistic ana ...
Rhodococcus sp.
J. Biol. Chem.
282
14300-14308
2007
-
1
1
1
2
-
-
2
1
-
-
-
-
1
-
-
1
-
-
-
-
-
3
-
2
-
-
-
2
-
-
-
-
-
-
-
-
1
1
-
1
2
-
-
-
-
2
1
-
-
-
-
-
-
1
-
-
-
-
3
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
665547
Horibata
Unique catabolic pathway of gl ...
Aiptasia sp., Bacillus sp. (in: Bacteria), Cellana sp., Cyanea nozakii, Entacmaea sp., Hydra vulgaris 105, Hydra vulgaris, Marphysa sp., Meretrix sp., Ruditapes sp.
J. Biol. Chem.
279
33379-33389
2004
2
-
1
-
-
-
1
-
-
5
3
-
-
18
-
-
1
-
-
4
12
-
25
1
18
-
-
-
-
9
-
-
-
-
1
-
2
-
1
-
-
-
-
-
1
-
-
-
5
3
-
-
-
-
1
-
4
12
-
25
1
-
-
-
-
9
-
-
1
-
-
-
-
-
-
646694
Horibata
Transglycosylation and reverse ...
Cyanea nozakii
J. Biochem.
130
263-268
2001
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
646695
Horibata
Purification, characterization ...
Cyanea nozakii
J. Biol. Chem.
275
31297-31304
2000
3
-
1
-
-
-
3
1
-
-
1
-
-
3
-
1
1
-
-
-
1
-
10
2
-
-
-
-
-
1
-
-
-
-
1
-
3
-
1
-
-
-
-
-
3
-
1
-
-
1
-
-
-
1
1
-
-
1
-
10
2
-
-
-
-
1
-
-
1
-
-
-
-
-
-
646702
Sakaguchi
Cloning and expression of gene ...
Rhodococcus sp., Rhodococcus sp. C9
J. Biochem.
128
145-152
2000
2
-
1
-
-
-
4
3
-
-
1
-
-
6
-
-
1
-
-
-
-
-
19
1
-
1
-
-
-
1
-
-
-
-
-
-
2
-
1
-
-
-
-
-
4
-
3
-
-
1
-
-
-
-
1
-
-
-
-
19
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
646700
Miura
Requirement for a different hy ...
Corynebacterium sp., Rhodococcus sp.
Glycobiology
9
957-960
1999
-
-
-
-
-
-
-
2
-
-
-
-
-
4
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
646701
Sakaguchi
The Glu residue in the conserv ...
Rhodococcus sp., Rhodococcus sp. C9
Biochem. Biophys. Res. Commun.
260
89-93
1999
-
-
1
-
4
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
2
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
2
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
646697
Ji
Effects of endoglycoceramidase ...
Rhodococcus sp.
Arch. Biochem. Biophys.
359
107-114
1998
1
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
1
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
646699
Mitsutake
Enzymatic synthesis of 14C-gly ...
Condylactis sp., Rhodococcus sp.
J. Biochem.
123
859-863
1998
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
646696
Izu
Molecular cloning, expression, ...
Rhodococcus sp.
J. Biol. Chem.
272
19846-19850
1997
-
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
11
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
11
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
646703
Yamamoto
Destruction of cholera toxin r ...
Corynebacterium sp.
Arch. Biochem. Biophys.
328
51-56
1996
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
646698
Ji
The hydrolysis of cell surface ...
Rhodococcus sp.
Glycobiology
5
343-350
1995
1
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
646689
Ashida
Transglycosylation activity of ...
Corynebacterium sp., Corynebacterium sp. A-809
Arch. Biochem. Biophys.
305
559-562
1993
-
-
-
-
-
-
-
-
1
-
-
-
-
4
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
646693
Ito
Kinetics of endoglycoceramidas ...
Rhodococcus sp.
Eur. J. Biochem.
218
645-649
1993
1
-
-
-
-
-
-
2
-
-
-
-
-
3
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
1
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
646688
Ashida
Purification and characterizat ...
Corynebacterium sp.
Eur. J. Biochem.
205
729-735
1992
2
-
-
-
-
1
2
1
3
-
2
-
-
3
-
-
1
-
-
1
1
1
6
1
-
-
-
2
-
1
-
2
-
-
-
-
2
-
-
-
-
-
1
-
2
-
1
3
-
2
-
-
-
-
1
-
1
1
1
6
1
-
-
2
-
1
-
2
-
-
-
-
-
-
-
646690
Ito
Activator proteins for glycosp ...
Rhodococcus sp.
J. Biol. Chem.
266
7919-7926
1991
2
-
-
-
-
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
2
-
-
-
-
1
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
646692
Ito
Conversion of endoglycoceramid ...
Rhodococcus sp.
J. Biochem.
110
328-332
1991
2
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
646691
Ito
Purification and characterizat ...
Rhodococcus sp.
J. Biol. Chem.
264
9510-9519
1989
4
-
-
-
-
2
6
4
-
-
5
-
-
3
-
-
1
-
-
2
1
2
20
1
-
-
-
1
-
2
1
1
-
-
2
-
4
-
-
-
-
-
2
-
6
-
4
-
-
5
-
-
-
-
1
-
2
1
2
20
1
-
-
1
-
2
1
1
2
-
-
-
-
-
-
646687
Ito
A novel glycosphingolipid-degr ...
Rhodococcus sp., Rhodococcus sp. G-74-2
J. Biol. Chem.
261
14278-14282
1986
1
-
-
-
-
-
3
-
-
-
-
-
-
3
-
-
1
-
-
1
1
-
25
-
-
-
-
-
-
1
-
1
-
-
-
-
1
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
1
-
1
1
-
25
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-