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Literature summary for 3.2.1.123 extracted from
- Transglycosylation-based fluorescent labeling of 6-gala series glycolipids by endogalactosylceramidase (2009), Glycobiology, 19, 797-807.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Rhodococcus equi |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodococcus equi | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni Sepharose 6 column chromatography and Superdex 200 gel filtration | Rhodococcus equi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 7-nitro-2,1,3-benzoxadiazole-pentanol + digalactosyldiacylglycerol | transglycosylation | Rhodococcus equi | ? | - |
? |  |
| Galalpha1-6Galbeta1-3-diacylglycerol + H2O | - |
Rhodococcus equi | ? | - |
? | |
| Galbeta1-1'-ceramide + H2O | - |
Rhodococcus equi | ? | - |
? | |
| Galbeta1-6Galbeta1-1'-ceramide + H2O | highest transglycosylation efficiency | Rhodococcus equi | ? | - |
? | |
| Galbeta1-6Galbeta1-6Galbeta1-1'-ceramide + H2O | - |
Rhodococcus equi | ? | - |
? | |
| Galbeta1-6Galbeta1-6Galbeta1-6Galbeta1-1'-ceramide + H2O | - |
Rhodococcus equi | ? | - |
? | |
| Glcbeta1-6Galbeta1-1'-ceramide + H2O | - |
Rhodococcus equi | ? | - |
? | |
| additional information | EGALC specifically hydrolyzes the Galbeta1-1'-ceramide linkage in 6-Gal series glycosphingolipids | Rhodococcus equi | ? | - |
? | |
| additional information | no activity towards Galbeta1-6Glcbeta1-1'-ceramide, Galbeta1-4Galbeta1-1'-ceramide, Galalpha1-1'ceramide, Glcbeta1-1'-ceramide, Galbeta1-4Glcbeta1-1'ceramide, Galalpha1-4Galbeta1-4Glcbeta1-1'-ceramide, and Galbeta1-3GalNAcbeta1-4(NeuAcalpha2-3)Galbeta1-4Glcbeta1-1'-ceramide | Rhodococcus equi | ? | - |
? | |
| trigalactosylceramide + H2O | - |
Rhodococcus equi | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| EGALC | - |
Rhodococcus equi |
| endogalactosylceramidase | - |
Rhodococcus equi |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
optimal temperature for transglycosylation by EGALC | Rhodococcus equi |
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Release 2023.1 (January 2023)
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