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Literature summary for 3.2.1.123 extracted from

  • Sakaguchi, K.; Okino, N.; Izu, H.; Ito, M.
    The Glu residue in the conserved Asn-Glu-Pro sequence of endoglycoceramidase is essential for enzymatic activity (1999), Biochem. Biophys. Res. Commun., 260, 89-93.
    View publication on PubMed
Search for more literature for 3.2.1.123

Cloned(Commentary)

Cloned (Comment) Organism
-
 Rhodococcus sp.

Protein Variants

Protein Variants Comment Organism
E224D strain C9, severely reduced enzyme activity Rhodococcus sp.
E224Q strain C9, severely reduced enzyme activity Rhodococcus sp.
E233D strain M-777, severely reduced enzyme activity Rhodococcus sp.
E233Q strain M-777, severely reduced enzyme activity Rhodococcus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
 x * 58000, SDS-PAGE Rhodococcus sp.

Organism

Organism UniProt Comment Textmining
Rhodococcus sp.
-
-
-
Rhodococcus sp.
-
 M-777
-
Rhodococcus sp. C9
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide 4-nitrobenzo-2-oxy-1,3-diazole + H2O i.e. NBD-GM1 Rhodococcus sp. Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc + 4-nitrobenzo-2-oxy-1,3-diazole ceramide
-
 ?
Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide 4-nitrobenzo-2-oxy-1,3-diazole + H2O i.e. NBD-GM1 Rhodococcus sp. C9 Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc + 4-nitrobenzo-2-oxy-1,3-diazole ceramide
-
 ?

Subunits

Subunits Comment Organism
? x * 58000, SDS-PAGE Rhodococcus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Rhodococcus sp.