Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.123 extracted from

  • Miura, Y.; Arai, T.; Ohtake, A.; Ito, M.; Yamamoto, K.; Yamagata, T.
    Requirement for a different hydrophobic moiety and reliable chromogenic substrate for endo-type glycosylceramidases (1999), Glycobiology, 9, 957-960.
    View publication on PubMed
Search for more literature for 3.2.1.123

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4
-
 2-N-hexadecanoylamino-4-nitrophenyl-O-beta-lactoside 4-nitrophenyllactoside derivative possessing an N-acyl chain, chromogenic substrate C16ampNPLac, pH 5 Corynebacterium sp.
2.9
-
 2-N-hexadecanoylamino-4-nitrophenyl-O-beta-lactoside 4-nitrophenyllactoside derivative possessing an N-acyl chain, chromogenic substrate C16ampNPLac, pH 5 Rhodococcus sp.

Organism

Organism UniProt Comment Textmining
Corynebacterium sp.
-
-
-
Rhodococcus sp.
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-N-hexadecanoylamino-4-nitrophenyl-O-beta-lactoside + H2O
-
 Corynebacterium sp. N-(2-hydroxy-5-nitrophenyl)hexadecanamide + beta-lactose
-
 ?
2-N-hexadecanoylamino-4-nitrophenyl-O-beta-lactoside + H2O derivative possessing an N-acyl chain, 4-nitrophenyllactoside chromogenic substrate C16ampNPLac Rhodococcus sp. N-(2-hydroxy-5-nitrophenyl)hexadecanamide + beta-lactose
-
 ?
additional information enzyme prefers lactosides with acylamide structures over simple n-alkyl lactosides as substrates Corynebacterium sp. ?
-
 ?
additional information enzyme prefers lactosides with acylamide structures over simple n-alkyl lactosides as substrates Rhodococcus sp. ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 assay at Rhodococcus sp.
6.5
-
 assay at Corynebacterium sp.