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Literature summary for 3.2.1.120 extracted from

  • Pauly, M.; Andersen, L.N.; Kauppinen, S.; Kofod, L.V.; York, W.S.; Albersheim, P.; Darvill, A.
    A xyloglucan-specific endo-beta-1,4-glucanase from Aspergillus aculeatus: expression cloning in yeast, purification and characterization of the recombinant enzyme (1999), Glycobiology, 9, 93-100.
    View publication on PubMed
Search for more literature for 3.2.1.120

Cloned(Commentary)

Cloned (Comment) Organism
expression in Aspergillus oryzae Aspergillus aculeatus

General Stability

General Stability Organism
very stable below 35°C Aspergillus aculeatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23600
-
 x * 23600, mass spectroscopy, x * 34000, SDS-PAGE, x * 23760, deduced from gene sequence Aspergillus aculeatus
23760
-
 x * 23600, mass spectroscopy, x * 34000, SDS-PAGE, x * 23760, deduced from gene sequence Aspergillus aculeatus
34000
-
 x * 23600, mass spectroscopy, x * 34000, SDS-PAGE, x * 23760, deduced from gene sequence Aspergillus aculeatus

Organism

Organism UniProt Comment Textmining
Aspergillus aculeatus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
no glycoprotein no N-glycosylation site Aspergillus aculeatus

Reaction

Reaction Comment Organism Reaction ID
R1-Glcbeta(1-4)Glcbeta[Xylbeta(1-6)Glcbeta(1-4)](1-4)Glcbeta(1-4)Glcbeta(1-4)-R2 + H2O = R1-Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)-R2 + 6-O-beta-D-xylopyranosyl-beta-D-glucopyranose stereoselective cleavage of substrate Aspergillus aculeatus
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
260
-
 21°C, pH 5.0 Aspergillus aculeatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
xyloglucan fragment oligosaccharide + H2O
-
 Aspergillus aculeatus isoprimeverose
-
 ?

Subunits

Subunits Comment Organism
? x * 23600, mass spectroscopy, x * 34000, SDS-PAGE, x * 23760, deduced from gene sequence Aspergillus aculeatus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 2 h, loss of 80% of activity Aspergillus aculeatus

pH Range

pH Minimum pH Maximum Comment Organism
3 3.8
-
 Aspergillus aculeatus