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Literature summary for 3.2.1.120 extracted from

  • Kato, Y.; Matsushita, J.; Kubodera, T.; Matsuda, K.
    A novel enzyme producing isoprimeverose from oligoxyloglucans of Aspergillus oryzae (1985), J. Biochem., 97, 801-810.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
Aspergillus oryzae
Fe2+
-
Aspergillus oryzae
Fe3+
-
Aspergillus oryzae
Hg2+
-
Aspergillus oryzae
SDS
-
Aspergillus oryzae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
115000
-
2 * 115000, SDS-PAGE Aspergillus oryzae
230000
-
gel filtration Aspergillus oryzae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
xyloglucan fragment oligosaccharide + H2O Aspergillus oryzae highly specific for isoprimeverose isoprimeverose is split off from the nonreducing end of the backbone of the substrate ?

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Aspergillus oryzae

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
Aspergillus oryzae
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
38.42
-
-
Aspergillus oryzae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
xyloglucan fragment oligosaccharide + H2O highly specific for Aspergillus oryzae isoprimeverose isoprimeverose is split off from the nonreducing end of the backbone of the substrate ?

Subunits

Subunits Comment Organism
dimer 2 * 115000, SDS-PAGE Aspergillus oryzae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Aspergillus oryzae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
stable up to Aspergillus oryzae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Aspergillus oryzae

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 7 45°C, 10 min, stable Aspergillus oryzae

pI Value

Organism Comment pI Value Maximum pI Value
Aspergillus oryzae isoelectric focusing
-
3.9