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Literature summary for 3.2.1.119 extracted from

  • Ahn, Y.O.; Saino, H.; Mizutani, M.; Shimizu, B.; Sakata, K.
    Vicianin hydrolase is a novel cyanogenic beta-glycosidase specific to beta-vicianoside (6-O-alpha-L-arabinopyranosyl-beta-D-glucopyranoside) in seeds of Vicia angustifolia (2007), Plant Cell Physiol., 48, 938-947.
    View publication on PubMed
Search for more literature for 3.2.1.119

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a GST fusion protein, full-length cDNA subcloned into the pFastBac1 plasmid for expression in insect cells using a baculovirus expression system Vicia sativa subsp. nigra

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55616
-
 1 * 56000, SDS-PAGE, 1 * 55616, sequence analysis Vicia sativa subsp. nigra
56000
-
 1 * 56000, SDS-PAGE, 1 * 55616, sequence analysis Vicia sativa subsp. nigra

Organism

Organism UniProt Comment Textmining
Vicia sativa subsp. nigra A2SY66
-
-

Purification (Commentary)

Purification (Comment) Organism
by gel filtration, 9.39fold Vicia sativa subsp. nigra

Source Tissue

Source Tissue Comment Organism Textmining
flower moderately present Vicia sativa subsp. nigra
-
leaf slightly present Vicia sativa subsp. nigra
-
root slightly present Vicia sativa subsp. nigra
-
seed abundantly present Vicia sativa subsp. nigra
-
stem slightly present Vicia sativa subsp. nigra
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.05
-
 crude extract Vicia sativa subsp. nigra
8.97
-
 recombinant enzyme, activity against vicianin Vicia sativa subsp. nigra
9.86
-
 native enzyme, purified, activity against vicianin Vicia sativa subsp. nigra

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-phenylethyl beta-primeveroside + H2O
-
 Vicia sativa subsp. nigra ?
-
 ?
furcatin + H2O recombinant enzyme expressed in Escherichia coli does not hydrolyze furcatin Vicia sativa subsp. nigra ?
-
 ?
additional information does not hydrolyze amygdalin, mandelonitrile-, 2-phenylethyl-, p-nitrophenyl beta-gentiobioside, p-nitrophenyl beta-D-xylopyranoside, p-nitrophenyl alpha-L-arabinopyranoside and p-nitrophenyl beta-D-galactopyranoside Vicia sativa subsp. nigra ?
-
 ?
p-nitrophenyl beta-primeveroside + H2O recombinant enzyme expressed in Escherichia coli does not hydrolyze p-nitrophenyl beta-primeveroside Vicia sativa subsp. nigra ?
-
 ?
prunasin + H2O shows 34% of its vicianin hydrolase activity with prunasin Vicia sativa subsp. nigra D-mandelonitrile + beta-D-glucose
-
 ?
vicianin + H2O best substrate, recombinant protein shows the same pattern of substrate specificity as the native enzyme Vicia sativa subsp. nigra vicianose + D-mandelonitrile
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 56000, SDS-PAGE, 1 * 55616, sequence analysis Vicia sativa subsp. nigra

Synonyms

Synonyms Comment Organism
vicianin hydrolase
-
 Vicia sativa subsp. nigra

pI Value

Organism Comment pI Value Maximum pI Value
Vicia sativa subsp. nigra
-
-
 8.69