Literature summary for 3.2.1.118 extracted from

Del Cueto, J.; Moller, B.L.; Dicenta, F.; Sanchez-Perez, R.
beta-Glucosidase activity in almond seeds (2018), Plant Physiol. Biochem., 126, 163-172 .

Search for more literature for 3.2.1.118

Cloned(Commentary)

Cloned (Comment)	Organism
RNA is extracted from the tegument, nucellus and cotyledon of one sweet and two bitter (D05-187 and S3067) almond genotypes throughout fruit ripening. Sequences of nine positive prunasin hydrolases are obtained from all of the genotypes by RT-PCR and cloning. The clones, from mid ripening stage, are expressed in a heterologous system in tobacco plants by agroinfiltration	Prunus dulcis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(R)-prunasin + H2O	Prunus dulcis	the enzyme is involved in the metabolic pathway for the synthesis and catabolism of the cyanogenic glucoside prunasin	D-glucose + mandelonitrile	-	?

Organism

Organism	UniProt	Comment	Textmining
Prunus dulcis	H9ZGE1	RNA is extracted from the tegument, nucellus and cotyledon of one sweet and two bitter (D05-187 and S3067) almond genotypes throughout fruit ripening. Sequences of nine positive prunasin hydrolases are obtained from all of the genotypes. Minor differences in the nucleotide and corresponding amino acid sequences are found between the nine prunasin hydrolases sequences. None of the differences can be related to the sweet or bitter flavour. Only PH691 from the D05-187 tegument shows reduced prunasin hydrolase activity; genotypes Lauranne (SkSk, sweet), D05-187 (sksk, bitter) and S3067 (sksk, bitter)	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
cotyledon	Ph691 appears in the tegument and nucellus of the bitter S3067 almond genotype at the beginning of the development period. It appears in the tegument, nucellus and cotyledon in both the bitter and sweet genotypes halfway through the development period, and only in the cotyledon of the bitter and sweet genotypes at the final development stage	Prunus dulcis	-
kernel	-	Prunus dulcis	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(R)-prunasin + H2O	-	Prunus dulcis	D-glucose + mandelonitrile	-	?
(R)-prunasin + H2O	the enzyme is involved in the metabolic pathway for the synthesis and catabolism of the cyanogenic glucoside prunasin	Prunus dulcis	D-glucose + mandelonitrile	-	?

Synonyms

Synonyms	Comment	Organism
Ph691	-	Prunus dulcis
prunasin hydrolase	-	Prunus dulcis

General Information

General Information	Comment	Organism
physiological function	the enzyme is involved in the metabolic pathway for the synthesis and catabolism of the cyanogenic glucoside prunasin	Prunus dulcis

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)