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Literature summary for 3.2.1.116 extracted from

  • Kamon, M.; Sumitani, J.; Tani, S.; Kawaguchi, T.; Kamon, M.; Sumitani, J.; Tani, S.; Kawaguchi, T.
    Characterization and gene cloning of a maltotriose-forming exo-amylase from Kitasatospora sp. MK-1785 (2015), Appl. Microbiol. Biotechnol., 99, 4743-4753 .
    View publication on PubMed
Search for more literature for 3.2.1.116

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Kitasatospora sp. MK-1785

General Stability

General Stability Organism
the enzyme is subjected to proteolysis at N-terminal region of the anterior starch-binding domain CBM20 in the C-terminal region Kitasatospora sp. MK-1785

Inhibitors

Inhibitors Comment Organism Structure
Ba2+
-
 Kitasatospora sp. MK-1785
Co2+
-
 Kitasatospora sp. MK-1785
Cu2+
-
 Kitasatospora sp. MK-1785
EDTA
-
 Kitasatospora sp. MK-1785
Mn2+
-
 Kitasatospora sp. MK-1785
Ni2+
-
 Kitasatospora sp. MK-1785
Zn2+
-
 Kitasatospora sp. MK-1785

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
23.8
-
 maltotetraose pH 6.0, 37°C Kitasatospora sp. MK-1785
33.9
-
 amylose pH 6.0, 37°C Kitasatospora sp. MK-1785
37.9
-
 maltopentaose pH 6.0, 37°C Kitasatospora sp. MK-1785

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ slightly enhances activity Kitasatospora sp. MK-1785
Li+ slightly enhances activity Kitasatospora sp. MK-1785
Mg2+ slightly enhances activity Kitasatospora sp. MK-1785
Sn2+ slightly enhances activity Kitasatospora sp. MK-1785

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
 SDS-PAGE Kitasatospora sp. MK-1785

Organism

Organism UniProt Comment Textmining
Kitasatospora sp. MK-1785 HW429890
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Kitasatospora sp. MK-1785

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylopectin + H2O 96.7% of the activity as compared to soluble starch Kitasatospora sp. MK-1785 maltotriose + ?
-
 ?
amylose + H2O 89.6% of the activity as compared to soluble starch Kitasatospora sp. MK-1785 maltotriose + ?
-
 ?
gamma-cyclodextrin + H2O 30.0% of the activity as compared to soluble starch Kitasatospora sp. MK-1785 maltotriose + maltose
-
 ?
glycogen + H2O 79.4% of the activity as compared to soluble starch Kitasatospora sp. MK-1785 maltotriose + ?
-
 ?
maltohexaose + H2O
-
 Kitasatospora sp. MK-1785 maltotriose + ?
-
 ?
maltopentaose + H2O
-
 Kitasatospora sp. MK-1785 maltotriose + maltose
-
 ?
maltotetraose + H2O
-
 Kitasatospora sp. MK-1785 maltotriose + glucose
-
 ?
additional information the enzyme exhibits transglycosylation activity toward phenolic and alcoholic compounds. It can not degrade pullulan Kitasatospora sp. MK-1785 ?
-
 ?
soluble starch + H2O the enzyme produces maltotriose as the principal product from starch Kitasatospora sp. MK-1785 maltotriose + ?
-
 ?

Subunits

Subunits Comment Organism
? x * 57000, SDS-PAGE Kitasatospora sp. MK-1785

Synonyms

Synonyms Comment Organism
G3Amy
-
 Kitasatospora sp. MK-1785
maltotriose-forming exo-amylase
-
 Kitasatospora sp. MK-1785

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Kitasatospora sp. MK-1785

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 70 40°C: about 50% of maximal activity, 70°C: about 40% of maximal activity Kitasatospora sp. MK-1785

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 the enzyme retains more than 80% activity after incubation for 20 min up to 50°C in the presence of 1 mM Ca2+ Kitasatospora sp. MK-1785

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
14.7
-
 maltotetraose pH 6.0, 37°C Kitasatospora sp. MK-1785
53.8
-
 amylose pH 6.0, 37°C Kitasatospora sp. MK-1785
58.1
-
 maltopentaose pH 6.0, 37°C Kitasatospora sp. MK-1785

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Kitasatospora sp. MK-1785

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 8 the enzyme retains more than 80% activity after 12 h incubation in the pH range from 4.0 to 8.0 Kitasatospora sp. MK-1785

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
617
-
 maltotetraose pH 6.0, 37°C Kitasatospora sp. MK-1785
1580
-
 amylose pH 6.0, 37°C Kitasatospora sp. MK-1785
1640
-
 maltopentaose pH 6.0, 37°C Kitasatospora sp. MK-1785