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Literature summary for 3.2.1.116 extracted from

  • Yang, C.; Liu, W.
    Purification and properties of a maltotriose-producing alpha-amylase from Thermobifida fusca (2004), Enzyme Microb. Technol., 35, 254-260.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Hg2+ 1 mM Thermobifida fusca

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Km-value for soluble starch is 0.88 mg/ml Thermobifida fusca

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
gel filtration Thermobifida fusca
64000
-
1 * 64000, SDS-PAGE Thermobifida fusca

Organism

Organism UniProt Comment Textmining
Thermobifida fusca
-
-
-
Thermobifida fusca NTU22
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Thermobifida fusca

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
raw sago starch + H2O
-
Thermobifida fusca maltotriose + ?
-
?
raw sago starch + H2O
-
Thermobifida fusca NTU22 maltotriose + ?
-
?
soluble starch + H2O
-
Thermobifida fusca maltotriose + ?
-
?
soluble starch + H2O
-
Thermobifida fusca NTU22 maltotriose + ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 64000, SDS-PAGE Thermobifida fusca

Synonyms

Synonyms Comment Organism
maltotriose-producing alpha-amylase
-
Thermobifida fusca

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Thermobifida fusca

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
3 h, about 70% of the activity remains Thermobifida fusca

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Thermobifida fusca