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Literature summary for 3.2.1.116 extracted from

  • Kobayashi, T.; Kanai, H.; Hayashi, T.; Akiba, T.; Akaboshi, R.; Horikoshi, K.
    Haloalkaliphilic maltotriose-forming alpha-amylase from the archaebacterium Natronococcus sp. strain Ah-36 (1992), J. Bacteriol., 174, 3439-3444.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.116

General Stability

General Stability Organism
0.5% soluble starch stabilizes Natronococcus sp.
2.5 M NaCl stabilizes Natronococcus sp.
activity is irreversibly lost at low ionic strength Natronococcus sp.
no loss of activity after dialysis Natronococcus sp.

Inhibitors

Inhibitors Comment Organism Structure
alpha-cyclodextrin competitive inhibitor, 0.25% Natronococcus sp.
beta-cyclodextrin competitive inhibitor, 0.025% Natronococcus sp.
Ca2+ 1 mM, 33% inhibition Natronococcus sp.
Hg2+ weak, 1 mM: 15% inhibition, 10 mM: 75% inhibition Natronococcus sp.
additional information not inhibited by 1 mM iodoacetic acid, 1 mM phenylmethylsulfonyl fluoride Natronococcus sp.
N-bromosuccinimide 1 mM, 73% inhibition Natronococcus sp.
Ni2+ 1 mM, 33% inhibition Natronococcus sp.
SDS 0.1%, 80% inhibition Natronococcus sp.
Urea 1 M, 74% inhibition Natronococcus sp.
ZnCl2 1 mM, complete inhibition Natronococcus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.8
-
 gamma-cyclodextrin pH 9, 50°C, Km-value shown as glucose equivalents Natronococcus sp.
19
-
 amylose pH 9, 50°C, DP, 17, Km-value shown as glucose equivalents Natronococcus sp.
25
-
 starch pH 9, 50°C, soluble, Km-value shown as glucose equivalents Natronococcus sp.
42
-
 glycogen pH 9, 50°C, from oyster, Km-value shown as glucose equivalents Natronococcus sp.
49
-
 amylopectin pH 9, 50°C, Km-value shown as glucose equivalents Natronococcus sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Natronococcus sp.
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
CsCl can partially substitute for NaCl at higher concentrations Natronococcus sp.
KCl can partially substitute for NaCl at higher concentrations Natronococcus sp.
NaCl requirement, maximum activity at 2.5 M NaCl, no activity below 1 M NaCl Natronococcus sp.
RbCl can partially substitute for NaCl at higher concentrations Natronococcus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
74000
-
 1 * 74000, SDS-PAGE Natronococcus sp.
76000
-
 gel filtration Natronococcus sp.

Organism

Organism UniProt Comment Textmining
Natronococcus sp.
-
-
-
Natronococcus sp. Ah-36
-
-
-

Purification (Commentary)

Purification (Comment) Organism
2000fold, in the presence of 2.5 M NaCl Natronococcus sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
160
-
 pH 9, 50°C Natronococcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3 glycogen + H2O from oyster, slow hydrolysis Natronococcus sp. 4 maltotriose additional small amounts of maltose and glucose, alpha-configuration ?
amylopectin + H2O
-
 Natronococcus sp. maltotriose additional small amounts of maltose and glucose, alpha-configuration ?
amylose + H2O
-
 Natronococcus sp. maltotriose
-
 ?
gamma-cyclodextrin + H2O
-
 Natronococcus sp. ?
-
 ?
maltoheptaose + H2O
-
 Natronococcus sp. maltotriose main product, additional small amounts of maltose and glucose, alpha-configuration ?
maltohexaose + H2O
-
 Natronococcus sp. maltotriose + ? alpha-configuration ?
maltohexaose + H2O
-
 Natronococcus sp. maltotriose + ? main product, additional small amounts of maltose and glucose ?
maltohexaose + H2O
-
 Natronococcus sp. Ah-36 maltotriose + ? alpha-configuration ?
maltohexaose + H2O
-
 Natronococcus sp. Ah-36 maltotriose + ? main product, additional small amounts of maltose and glucose ?
maltooligosaccharide + H2O ranging from maltotetraose to maltoheptaose, not: maltotriose, maltose Natronococcus sp. maltotriose alpha-configuration ?
maltopentaose + H2O
-
 Natronococcus sp. maltotriose + D-maltose alpha-configuration ?
maltopentaose + H2O
-
 Natronococcus sp. maltotriose + D-maltose additional small amounts of glucose ?
maltotetraose + H2O
-
 Natronococcus sp. maltotriose + D-glucose alpha-configuration, additional product: D-maltose ?
additional information not: alpha-cyclodextrin, pullulan Natronococcus sp. ?
-
 ?
additional information not: maltotriose, maltose, beta-cyclodextrin Natronococcus sp. ?
-
 ?
additional information enzyme has transferase activity using maltopentaose or maltotetraose as substrates Natronococcus sp. ?
-
 ?
additional information not: alpha-cyclodextrin, pullulan Natronococcus sp. Ah-36 ?
-
 ?
additional information not: maltotriose, maltose, beta-cyclodextrin Natronococcus sp. Ah-36 ?
-
 ?
additional information enzyme has transferase activity using maltopentaose or maltotetraose as substrates Natronococcus sp. Ah-36 ?
-
 ?
short-chain amylose + H2O DP, 17, most rapid hydrolysis Natronococcus sp. maltotriose alpha-configuration ?
short-chain amylose + H2O DP, 17, most rapid hydrolysis Natronococcus sp. maltotriose additional small amounts of maltose and glucose ?
starch + H2O soluble Natronococcus sp. maltotriose + ? additional small amounts of maltose and glucose, alpha-configuration ?

Subunits

Subunits Comment Organism
monomer 1 * 74000, SDS-PAGE Natronococcus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 assay at Natronococcus sp.
55
-
 pH 9, 30 min, in the presence of 2.5 M NaCl Natronococcus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 soluble starch stabilizes against thermal inactivation, Ca2+ does not enhance thermal stability Natronococcus sp.
50
-
 30 min, 2.5 M NaCl, pH 4.2: 80% loss of activity, pH 5.0: 44% loss of activity, pH 6.0-8.6: stable, pH 10.5: 30% loss of activity Natronococcus sp.
55
-
 pH 8.0, 30 min, 2.5 M NaCl, 50% loss of activity, no loss of activity in the presence of 0.5% soluble starch Natronococcus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.7
-
 in the presence of 2.5 M NaCl Natronococcus sp.
9
-
 assay at Natronococcus sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.5
-
 30 min, 50°C, 2.5 M NaCl, 0.5% soluble starch, 80% loss of activity Natronococcus sp.
4.2
-
 30 min, 50°C, 2.5 M NaCl, 80% loss of activity Natronococcus sp.
5
-
 30 min, 50°C, 2.5 M NaCl, 44% loss of activity Natronococcus sp.
6 10 30 min, 50°C, 2.5 M NaCl, 0.5% soluble starch, stable Natronococcus sp.
6 8.6 30 min, 50°C, 2.5 M NaCl, stable Natronococcus sp.
10.5
-
 30 min, 50°C, 2.5 M NaCl, 30% loss of activity Natronococcus sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.41
-
 beta-cyclodextrin pH 9, 50°C, 0.025% Natronococcus sp.
1.63
-
 alpha-cyclodextrin pH 9, 50°C, 0.25% Natronococcus sp.