Literature summary for 3.2.1.114 extracted from

Kumar, N.S.; Kuntz, D.A.; Wen, X.; Pinto, B.M.; Rose, D.R.
Binding of sulfonium-ion analogues of di-epi-swainsonine and 8-epi-lentiginosine to Drosophila Golgi alpha-mannosidase II: The role of water in inhibitor binding (2008), Proteins, 71, 1484-1496.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
by hanging-drop vapor diffusion method, GMII in complex with inhibitors di-epi-swainsonine and 8-epi-lentiginosine, both adopt a 3C6/E7 conformation, water molecule substructure in the active site plays a significant role in dictating inhibitory activity	Drosophila melanogaster

Inhibitors

Inhibitors	Comment	Organism	Structure
(1R,2S,8aS)-1,2-dihydroxyoctahydrothieno[1,2-a]thiopyranium chloride	good inhibitor, lacks a hydroxyl group at C-5, more than 140fold better inhibitor of GMII than di-epi-swainsonine	Drosophila melanogaster
8-epi-lentiginosine	-	Drosophila melanogaster
di-epi-swainsonine	weak inhibitor	Drosophila melanogaster
Mannostatin A	-	Drosophila melanogaster
swainsonine	-	Drosophila melanogaster

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	di-epi-swainsonine has only a single hydroxyl group at C-8 interacting with the zinc ion of the enzyme	Drosophila melanogaster

Organism

Organism	UniProt	Comment	Textmining
Drosophila melanogaster	Q24451	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Drosophila melanogaster

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl alpha-D-mannoside + H2O	-	Drosophila melanogaster	4-nitrophenol + alpha-D-mannose	-	?

Synonyms

Synonyms	Comment	Organism
GmII	-	Drosophila melanogaster
Golgi alpha-mannosidase II	-	Drosophila melanogaster

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.014	-	-	Drosophila melanogaster	8-epi-lentiginosine
2	-	-	Drosophila melanogaster	di-epi-swainsonine

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Release 2023.1 (January 2023)