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Literature summary for 3.2.1.114 extracted from

  • Akama, T.O.; Nakagawa, H.; Wong, N.K.; Sutton-Smith, M.; Dell, A.; Morris, H.R.; Nakayama, J.; Nishimura, S.; Pai, A.; Moremen, K.W.; Marth, J.D.; Fukuda, M.N.
    Essential and mutually compensatory roles of {alpha}-mannosidase II and {alpha}-mannosidase IIx in N-glycan processing in vivo in mice (2006), Proc. Natl. Acad. Sci. USA, 103, 8983-8988.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.114

Application

Application Comment Organism
medicine alpha-mannosidase IIx is an isozyme of MII. Either MII or alpha-mannosidase IIx can biochemically compensate for the deficiency of the other in vivo, and either of two is required for late embryonic and early postnatal development Mus musculus

Cloned(Commentary)

Cloned (Comment) Organism
cDNA fragments encoding full-length enzymes subcloned into the pcDNA3.1 mammalian expression vector. cDNA fragments encoding soluble forms of enzymes subcloned into pcDNA-HSH. Full-length alpha-mannosidase IIx expressed in MII/alpha-mannosidase IIx double-null fibroblasts. Soluble recombinant alpha-mannosidase IIx expressed in COS cells Mus musculus

Protein Variants

Protein Variants Comment Organism
additional information MII/alpha-mannosidase IIx double knockout mice, completely lack complex-type N-glycans, some double-nulls die between embryonic days 15.5 and 18.5, but most survive until shortly after birth and die of respiratory failure. Alpha-mannosidase IIx-null mice are mainly complex-type and also contain high-mannose-type but not significant levels of hybrid-type N-glycans. In contrast, MII-null embryos show high levels of hybrid-type N-glycans and reduced levels of complex-type N-glycans Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Mus musculus MII and alpha-mannosidase IIx function in N-glycan processing in a similar manner ?
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 ?

Organism

Organism UniProt Comment Textmining
Mus musculus
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Source Tissue

Source Tissue Comment Organism Textmining
embryo
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 Mus musculus
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GlcNAc(Man)5GlcNAc2 + H2O alpha-mannosidase IIx protein Mus musculus GlcNAc(Man)3GlcNAc2 + D-mannose
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 ?
additional information MII and alpha-mannosidase IIx function in N-glycan processing in a similar manner Mus musculus ?
-
 ?
additional information MII and alpha-mannosidase IIx function in N-glycan processing in a similar manner. Alpha-mannosidase IIx shows no reactivity to high-mannose-type carbohydrates Mus musculus ?
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 ?

Synonyms

Synonyms Comment Organism
alpha-mannosidase II
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 Mus musculus
alpha-mannosidase IIx
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 Mus musculus
MII
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 Mus musculus