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Literature summary for 3.2.1.114 extracted from
- The production, purification and characterization of two novel alpha-D-mannosidases from Aspergillus phoenicis (2005), Carbohydr. Res., 340, 609-617.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 10 mM | Aspergillus phoenicis |  |
| Mn2+ | - |
Aspergillus phoenicis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 14 | - |
alpha-D-Man-(1,6)-D-Man | - |
Aspergillus phoenicis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 1 mM | Aspergillus phoenicis | |
| additional information | does not need Ca2+ or any other metal cofactor of those tested | Aspergillus phoenicis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 74000 | - |
SDS-PAGE | Aspergillus phoenicis |
| 81000 | - |
native-PAGE | Aspergillus phoenicis |
| 97000 | - |
SDS-PAGE | Aspergillus phoenicis |
| 110000 | - |
native-PAGE | Aspergillus phoenicis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus phoenicis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| anion-exchange chromatography, chromatofocusing chromatography, gel filtration | Aspergillus phoenicis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-D-Man-(1,6)-D-Man + H2O | - |
Aspergillus phoenicis | alpha-D-mannopyranose | - |
? | |
| alpha-D-Manp-(1,3)-D-Manp + H2O | - |
Aspergillus phoenicis | alpha-D-mannopyranose | - |
? | |
| alpha-D-Manp-(1,6)-D-Manp + H2O | - |
Aspergillus phoenicis | alpha-D-mannopyranose | - |
? | |
| baker's yeast mannan + H2O | - |
Aspergillus phoenicis | ? | - |
? | |
| p-nitrophenyl-alpha-D-mannopyranoside + H2O | - |
Aspergillus phoenicis | p-nitrophenol + alpha-D-mannopyranose | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 1,3(1,6)-alpha-D-mannosidase | - |
Aspergillus phoenicis |
| 1,6-alpha-D-mannosidase | - |
Aspergillus phoenicis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Aspergillus phoenicis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 4.5 | - |
Aspergillus phoenicis |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Aspergillus phoenicis | for the first isoform of the enzyme, isoelectric focusing | - |
4.3 |
| Aspergillus phoenicis | method not mentioned | - |
4.6 |
| Aspergillus phoenicis | for the second isoform of the enzyme, isoelectric focusing | - |
4.8 |
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