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Literature summary for 3.2.1.114 extracted from
- Swainsonine inhibits the biosynthesis of complex glycoproteins by inhibition of Golgi mannosidase II (1982), J. Biol. Chem., 257, 7936-7939.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| swainsonine | 200 nM, 50% inhibition; i.e. (1S,2R,8R,8aR)-1,2,8-trihydroxyoctahydroindolizidine; very strong | Rattus norvegicus |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| Golgi membrane | - |
Rattus norvegicus | 139 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Rattus norvegicus | asparagine-linked glycoprotein biosynthesis | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
male Wistar rats | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl alpha-D-mannoside + H2O | - |
Rattus norvegicus | 4-nitrophenol + alpha-D-mannose | - |
? | |
| GlcNAc(Man)5GlcNAc + 2 H2O | removes the exposed alpha-1,3- and alpha-1,6-mannosyl residues | Rattus norvegicus | GlcNAc(Man)3GlcNAc + 2 alpha-D-mannose | - |
? | |
| additional information | not: (Man)9GlcNAc | Rattus norvegicus | ? | - |
? | |
| additional information | not: GlcNAc(Man)3GlcNAc | Rattus norvegicus | ? | - |
? | |
| additional information | not: (Man)5GlcNAc | Rattus norvegicus | ? | - |
? | |
| additional information | asparagine-linked glycoprotein biosynthesis | Rattus norvegicus | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
assay at | Rattus norvegicus |
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Release 2023.1 (January 2023)
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