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Literature summary for 3.2.1.114 extracted from

  • Amano, J.; Kobata, A.
    Purification and characterization of a novel alpha-mannosidase from Aspergillus saitoi (1986), J. Biochem., 99, 1645-1654.
    View publication on PubMed
Search for more literature for 3.2.1.114

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 1 mM, 50% inhibition Aspergillus phoenicis
additional information not inhibited by EDTA Aspergillus phoenicis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.25
-
 Manalpha(1,6)(Manalpha(1,3))Manbeta(1,4)GlcNAcbeta(1,4)GlcNAc NaBH4 reduced Aspergillus phoenicis
1.25
-
 Manalpha(1,6)(Manalpha(1,3))Manbeta(1,4)GlcNAcbeta(1,4)GlcNAc pH 5, 37°C Aspergillus phoenicis

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ strongly activates, 1 mM, 13fold Aspergillus phoenicis
additional information not activated by Zn2+ Aspergillus phoenicis
additional information not activated by Ba2+ Aspergillus phoenicis
additional information not activated by Mg2+, Mn2+ Aspergillus phoenicis

Organism

Organism UniProt Comment Textmining
Aspergillus phoenicis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
1000fold Aspergillus phoenicis

Source Tissue

Source Tissue Comment Organism Textmining
mycelium freeze-dried mixture of mycelium and its culture medium, commercially available as Morushin Aspergillus phoenicis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
38000
-
-
 Aspergillus phoenicis

Storage Stability

Storage Stability Organism
-20°C or 4°C, 4 months, less than 5% loss of activity Aspergillus phoenicis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + H2O NaBH4 reduced, poor substrate Aspergillus phoenicis ?
-
 ?
D-Manalpha(1-3)Manbeta(1-4)GlcNAc + H2O NaBH4 reduced, good substrate Aspergillus phoenicis D-Manbeta(1-4)GlcNAc + alpha-D-mannose NaBH4 reduced ?
D-Manalpha(1-6)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O NaBH4 reduced, at lower rate than Manalpha(1-3)Manbeta(1-4)GlcNAc hydrolysis Aspergillus phoenicis D-Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + alpha-D-mannose NaBH4 reduced ?
GlcNAcbeta(1-2)Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O NaBH4 reduced, hydrolysis at equal rate as Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc, galactosyl and sialyl-galactosyl derivatives are substrates, too Aspergillus phoenicis ?
-
 ?
GlcNAcbeta(1-6)(GlcNAcbeta(1-2))Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O NaBH4 reduced, rate at 50% of Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc hydrolysis Aspergillus phoenicis ?
-
 ?
Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O NaBH4 reduced Aspergillus phoenicis Manalpha(1-6)(Manalpha(1-3))D-Manalpha(1-6)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + alpha-D-mannose NaBH4 reduced ?
Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O NaBH4 reduced, cleaves the Manalpha(1-6)Man linkage only after its Manalpha(1-3) residue is removed Aspergillus phoenicis Manalpha(1-6)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + alpha-D-mannose NaBH4 reduced, no product: Manalpha(1-3)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc ?
additional information not: 4-nitrophenyl alpha-mannoside Aspergillus phoenicis ?
-
 ?
additional information not: D-Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(GlcNAcbeta(1-4))[GlcNAcbeta(1-4)(GlcNAcbeta(1-2))Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc, GlcNAcbeta(1-2)Manalpha(1-3)(Manalpha(1-6))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc, GlcNAcbeta(1-4)Manalpha(1-3)(Manalpha(1-6))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc, all sodium borate reduced Aspergillus phoenicis ?
-
 ?
additional information substrate specificity, hydrolysis mechanism of branched oligosaccharides Aspergillus phoenicis ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Aspergillus phoenicis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Aspergillus phoenicis

pH Range

pH Minimum pH Maximum Comment Organism
3.5 6 pH 3.5: more than 70% of maximal activity, pH 6: about half-maximal activity Aspergillus phoenicis