Activating Compound | Comment | Organism | Structure |
---|---|---|---|
NaCl | 0.1 M, required for optimal activity | Prunus dulcis |
General Stability | Organism |
---|---|
major loss of activity by ion-exchange chromatography | Prunus dulcis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.095 | - |
lacto-N-fucopentaitol III | - |
Prunus dulcis | |
0.101 | - |
lacto-N-fucopentaitol II | pH 5.0, 37°C | Prunus dulcis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Prunus dulcis | the enzyme hydrolyzes fucose alpha-1,3 or alpha-1,4-linkages to N-acteylglucosamine | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Prunus dulcis | - |
almond | - |
Purification (Comment) | Organism |
---|---|
partial | Prunus dulcis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
emulsin | - |
Prunus dulcis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00146 | - |
- |
Prunus dulcis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
lacto-N-difucohexaitol + H2O | poor substrate | Prunus dulcis | ? | - |
? | |
lacto-N-fucopentaitol + H2O | lacto-N-fucopentaitol II or III, no substrate: lacto-N-fucopentaitol I | Prunus dulcis | ? | - |
? | |
lacto-N-fucopentaitol II + H2O | - |
Prunus dulcis | ? | - |
? | |
lacto-N-fucopentaitol III + H2O | - |
Prunus dulcis | ? | - |
? | |
additional information | no hydrolysis of fucose alpha-1,6 or alpha-1,2-linkages to N-acetylglucosamine, as in IgG glycopeptides or 2'-fucosyllactitol | Prunus dulcis | ? | - |
? | |
additional information | the enzyme hydrolyzes fucose alpha-1,3 or alpha-1,4-linkages to N-acteylglucosamine | Prunus dulcis | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Prunus dulcis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 6.8 | about half-maximal activity at pH 4 and 6.8 | Prunus dulcis |