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Literature summary for 3.2.1.11 extracted from
- Biochemical characterization of dextranase from Arthrobacter oxydans and its cloning and expression in Escherichia coli (2010), Food Sci. Biotechnol., 19, 757-762.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene aodex, DNA and amino acid sequence determination and analysis, expression of the His-tagged enzyme in Escherichia coli | Pseudarthrobacter oxydans |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 68300 | - |
x * 71000, SDS-PAGE, x * 68300, about, sequence calculation | Pseudarthrobacter oxydans |
| 71000 | - |
x * 71000, SDS-PAGE, x * 68300, about, sequence calculation | Pseudarthrobacter oxydans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Pseudarthrobacter oxydans | AODex treatment of stale sugar cane juice results in a yield of square and light-colored sugar crystals | ? | - |
? |  |
| additional information | Pseudarthrobacter oxydans DSM 20119T | AODex treatment of stale sugar cane juice results in a yield of square and light-colored sugar crystals | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudarthrobacter oxydans | A9UKG4 | isolated from sugar-cane farm soil, gene aodex | - |
| Pseudarthrobacter oxydans DSM 20119T | A9UKG4 | isolated from sugar-cane farm soil, gene aodex | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme by anion exchange chromatography, recombinant His-tagged enzyme 16fold from Escherichia coli by nickel affinity chromatography | Pseudarthrobacter oxydans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | AODex treatment of stale sugar cane juice results in a yield of square and light-colored sugar crystals | Pseudarthrobacter oxydans | ? | - |
? | |
| additional information | AODex treatment of stale sugar cane juice results in a yield of square and light-colored sugar crystals | Pseudarthrobacter oxydans DSM 20119T | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 71000, SDS-PAGE, x * 68300, about, sequence calculation | Pseudarthrobacter oxydans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AODex | - |
Pseudarthrobacter oxydans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Pseudarthrobacter oxydans |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 60 | over 80% activity retained within the range of 37-50°C, 50% of maximal activity at 25°C and 60°C | Pseudarthrobacter oxydans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
purified enzyme, 3 h, stable below | Pseudarthrobacter oxydans |
| 55 | - |
purified enzyme, 3 h, 80% activity remaining | Pseudarthrobacter oxydans |
| 60 | - |
purified enzyme, 3 h, inactivation | Pseudarthrobacter oxydans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Pseudarthrobacter oxydans |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 8.3 | 70-100% activity at pH 6.0-8.0, 50% activity at pH 5.5 and pH 8.3 | Pseudarthrobacter oxydans |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
purified enzyme, 3 h, 40% activity remaining | Pseudarthrobacter oxydans |
| 6 | - |
purified enzyme, 3 h, 70% activity remaining | Pseudarthrobacter oxydans |
| 7 | 7.5 | purified enzyme, 3 h, 100% activity | Pseudarthrobacter oxydans |
| 8 | - |
purified enzyme, 3 h, 90% activity remaining | Pseudarthrobacter oxydans |
| 9 | - |
purified enzyme, 3 h, 60% activity remaining | Pseudarthrobacter oxydans |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | dextranase hydrolyzes the alpha-1,6 glucosidic bond of dextran to release isomaltooligosaccharides, as endo-dextranase, produced by various microorganisms including bacteria, yeast, and fungi | Pseudarthrobacter oxydans |
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