Protein Variants | Comment | Organism |
---|---|---|
additional information | immobilization of dextranase on several carriers by entrapment and covalent binding with cross-linking. Entrapment in sodium alginate with Ca2+. Dextranase immobilized on bovine serum albumin with a cross-linking agent shows the highest activity and considerable immobilization yield of 66.7%. The optimum pH of the immobilized enzyme is shifted to pH 6.0 as compared to the free enzyme pH 5.5. The optimum temperature of the reaction is at 60°C for both free and immobilized enzyme. Thermal and pH stability are significantly improved by the immobilization process. Km of the immobilized dextranase is higher than that of the free dextranase, while Vmax of the immobilized enzyme is lower than that of the free dextranase. The immobilized enzyme is able to retain 76% of the initial catalytic activity after 5.0 cycles | Aspergillus subolivaceus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 93% inhibition of the free enzyme, 56.5% inhibition of the immobilized enzyme | Aspergillus subolivaceus | |
Fe2+ | 41.5% inhibition of the free enzyme, 18% inhibition of the immobilized enzyme | Aspergillus subolivaceus | |
Hg2+ | complete inhibition of the free enzyme and the immobilized enzyme | Aspergillus subolivaceus | |
Mn2+ | 23% inhibition of the free enzyme, 6% inhibition of the immobilized enzyme | Aspergillus subolivaceus | |
Na+ | 85% inhibition of the free enzyme, 60% inhibition of the immobilized enzyme | Aspergillus subolivaceus | |
Zn2+ | 21% inhibition of the free enzyme, 8% inhibition of the immobilized enzyme | Aspergillus subolivaceus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no effects on enzyme activity by K+, Mg2+, and Ca2+ on both free and immobilized enzymes | Aspergillus subolivaceus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus subolivaceus | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1560 | - |
bovine serum albumin immobilized enzyme, pH 5.5, 60°C | Aspergillus subolivaceus |
8130 | - |
free enzyme, pH 5.5, 60°C | Aspergillus subolivaceus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
both free and immobilized enzyme | Aspergillus subolivaceus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
half-life of the free enzyme is 331.15 min, and of the immobilized enzyme 431.8 min | Aspergillus subolivaceus |
70 | - |
half-life of the free enzyme is 49.22 min, and of the immobilized enzyme 70.15 min | Aspergillus subolivaceus |
80 | - |
half-life of the free enzyme is 4.21 min, and of the immobilized enzyme 30.43 min | Aspergillus subolivaceus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
free enzyme | Aspergillus subolivaceus |
6 | - |
immobilized enzyme | Aspergillus subolivaceus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 8 | activity range of both free and immobilized enzyme | Aspergillus subolivaceus |