Any feedback?
Literature summary for 3.2.1.11 extracted from
- Rational introduction of disulfide bond to enhance optimal temperature of Lipomyces starkeyi alpha-dextranase expressed in Pichia pastoris (2009), J. Microbiol. Biotechnol., 19, 1506-1513.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Pichia pastoris strain GS115 | Lipomyces starkeyi |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D279C/S289C | the mutant shows a more than 13°C improvement on its optimal temperature compared to the wild type enzyme | Lipomyces starkeyi |
| D279C/S289C | the mutant shows a more than 3°C improvement on its optimal temperature compared to the wild type enzyme | Lipomyces starkeyi |
| T245C/N248C | the mutant shows a more than 3°C improvement on its optimal temperature compared to the wild type enzyme | Lipomyces starkeyi |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 67200 | - |
calculated from amino acid sequence | Lipomyces starkeyi |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dextran + H2O | Lipomyces starkeyi | - |
oligosaccharides | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lipomyces starkeyi | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 13.66 | - |
wild type enzyme, at pH 4.5, 30°C | Lipomyces starkeyi |
| 14.93 | - |
mutant enzyme D279C/S289C, at pH 4.5, 43°C | Lipomyces starkeyi |
| 25.6 | - |
mutant enzyme T245C/N248C, at pH 5.0, 33°C | Lipomyces starkeyi |
| 44.52 | - |
mutant enzyme D279C/S289C, at pH 5.0, 33°C | Lipomyces starkeyi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dextran + H2O | - |
Lipomyces starkeyi | oligosaccharides | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha-dextranase | - |
Lipomyces starkeyi |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Lipomyces starkeyi |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
- |
Lipomyces starkeyi |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
