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Literature summary for 3.2.1.11 extracted from

  • Tamura, H.; Yamada, A.; Kato, H.
    Identification and characterization of a dextranase gene of Streptococcus criceti (2007), Microbiol. Immunol., 51, 721-732.
    View publication on PubMed
Search for more literature for 3.2.1.11

Cloned(Commentary)

Cloned (Comment) Organism
into vector pET-21a(+), recombinant form with six histidine residues tagged in the C-terminus expressed in Escherichia coli strain BL21(DE3) Streptococcus criceti

Protein Variants

Protein Variants Comment Organism
D453E loss of dextranase activity Streptococcus criceti
additional information deletion mutant of the C-terminal region can hydrolyze blue dextrans. Double mutant W793L and deletion mutant of the C-terminal region has no dextranase activity Streptococcus criceti
W793L loss of dextranase activity Streptococcus criceti

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
97000
-
 SDS-PAGE, dextranase band from whole-cell extract Streptococcus criceti
97000
-
 strain MT8148, SDS-PAGE Streptococcus mutans
100000
-
 strain 6715DP, SDS-PAGE Streptococcus sobrinus
115000
-
 SDS-PAGE, dextranase band from whole-cell extract Streptococcus criceti
128100
-
 sequence analysis Streptococcus criceti
150000
-
 SDS-PAGE, dextranase band from whole-cell extract Streptococcus criceti
170000
-
 SDS-PAGE, dextranase band from whole-cell extract Streptococcus criceti
170000
-
 strain M36, SDS-PAGE Streptococcus salivarius
180000
-
 SDS-PAGE, dextranase band from whole-cell extract Streptococcus criceti
190000
-
 strain M36, SDS-PAGE Streptococcus salivarius
200000
-
 SDS-PAGE, dextranase band from whole-cell extract Streptococcus criceti
220000
-
 strain 6715DP, SDS-PAGE Streptococcus sobrinus

Organism

Organism UniProt Comment Textmining
no activity in Streptococcus anginosus
-
-
-
no activity in Streptococcus anginosus NCTC10713
-
-
-
no activity in Streptococcus constellatus
-
 strain ATCC27823
-
no activity in Streptococcus constellatus ATCC 27823
-
 strain ATCC27823
-
no activity in Streptococcus gordonii
-
 strain ATCC10558
-
no activity in Streptococcus gordonii ATCC 10558
-
 strain ATCC10558
-
no activity in Streptococcus intermedius
-
-
-
no activity in Streptococcus intermedius GAI-1157
-
-
-
no activity in Streptococcus oralis
-
 strain ATCC10557
-
no activity in Streptococcus oralis ATCC 10557
-
 strain ATCC10557
-
no activity in Streptococcus sanguinis
-
 strain ATCC10556
-
no activity in Streptococcus sanguinis ATCC 10556
-
 strain ATCC10556
-
Streptococcus criceti
-
 serotype A
-
Streptococcus criceti A4F2S5 strain ATCC19642, serotype a
-
Streptococcus criceti Q1MVS0 serotype A
-
Streptococcus criceti ATCC 19642 A4F2S5 strain ATCC19642, serotype a
-
Streptococcus criceti E49 Q1MVS0 serotype A
-
Streptococcus criceti HS-1
-
 serotype A
-
Streptococcus downei Q8VLP4 serotype h
-
Streptococcus downei MFe28 Q8VLP4 serotype h
-
Streptococcus mutans Q54443 strain MT8148, serotype c, and strain MT8148, serotype f
-
Streptococcus ratti Q76EQ4 serotype b
-
Streptococcus ratti BHT Q76EQ4 serotype b
-
Streptococcus salivarius
-
-
-
Streptococcus salivarius M36
-
-
-
Streptococcus sobrinus P39653 strain SL1, serotype d, and strain 6715DP, serotype g
-

Purification (Commentary)

Purification (Comment) Organism
recombinant form with six histidine residues tagged in the C-terminus partially purified on Ni-NTA column Streptococcus criceti

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
blue dextran brain heart infusion agar plates
-
 Streptococcus salivarius ?
-
 ?
blue dextran brain heart infusion agar plates
-
 Streptococcus ratti ?
-
 ?
blue dextran brain heart infusion agar plates
-
 Streptococcus mutans ?
-
 ?
blue dextran brain heart infusion agar plates
-
 Streptococcus sobrinus ?
-
 ?
blue dextran brain heart infusion agar plates
-
 Streptococcus downei ?
-
 ?
blue dextran brain heart infusion agar plates
-
 Streptococcus downei MFe28 ?
-
 ?
blue dextran brain heart infusion agar plates
-
 Streptococcus salivarius M36 ?
-
 ?
blue dextran brain heart infusion agar plates
-
 Streptococcus ratti BHT ?
-
 ?
blue-dextran + H2O Asp-453 and Trp-793 residues are critical for enzyme activity Streptococcus salivarius ?
-
 ?
blue-dextran + H2O Asp-453 and Trp-793 residues are critical for enzyme activity Streptococcus criceti ?
-
 ?
blue-dextran + H2O Asp-453 and Trp-793 residues are critical for enzyme activity Streptococcus ratti ?
-
 ?
blue-dextran + H2O Asp-453 and Trp-793 residues are critical for enzyme activity Streptococcus mutans ?
-
 ?
blue-dextran + H2O Asp-453 and Trp-793 residues are critical for enzyme activity Streptococcus sobrinus ?
-
 ?
blue-dextran + H2O Asp-453 and Trp-793 residues are critical for enzyme activity Streptococcus downei ?
-
 ?
blue-dextran + H2O Asp-453 and Trp-793 residues are critical for enzyme activity Streptococcus criceti ATCC 19642 ?
-
 ?
blue-dextran + H2O Asp-453 and Trp-793 residues are critical for enzyme activity Streptococcus criceti E49 ?
-
 ?
blue-dextran + H2O Asp-453 and Trp-793 residues are critical for enzyme activity Streptococcus criceti HS-1 ?
-
 ?
blue-dextran + H2O Asp-453 and Trp-793 residues are critical for enzyme activity Streptococcus downei MFe28 ?
-
 ?
blue-dextran + H2O Asp-453 and Trp-793 residues are critical for enzyme activity Streptococcus salivarius M36 ?
-
 ?
blue-dextran + H2O Asp-453 and Trp-793 residues are critical for enzyme activity Streptococcus ratti BHT ?
-
 ?
additional information no activity on blue dextran brain heart infusion agar plates Streptococcus criceti ?
-
 ?
additional information no activity on blue dextran brain heart infusion agar plates Streptococcus criceti ATCC 19642 ?
-
 ?
additional information no activity on blue dextran brain heart infusion agar plates Streptococcus criceti E49 ?
-
 ?
additional information no activity on blue dextran brain heart infusion agar plates Streptococcus criceti HS-1 ?
-
 ?

pI Value

Organism Comment pI Value Maximum pI Value
Streptococcus criceti sequence analysis
-
 4.2