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Literature summary for 3.2.1.11 extracted from
- Immobilization of dextranase from Chaetomium erraticum (2007), J. Biotechnol., 131, 440-447.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | bentonite carriers exhibit high monolayer protein and activity loads at pH 4.0 and after pH-shift to 5.4. If dextranase is adsorbed at pH 4.1 and hydroxyapatite is washed several times with buffer at pH 5.4 reveals highest activity yield so far (28%). Other selected carriers used are hydroxyapatite and Streamline DEAE. In general, immobilization yields are low and in case of DEAE the rather high particle size is problematic. Porous Eupergit differs only moderately in activity using low molecular weight substrate, whereas macroporous Eupergit reveals enhanced activity for high molecular weight substrates. Activation of the carbohydrate moiety of dextranase by metaperiodate results in active silica gels, whereas the activation of alginate via Woodwards reagent K exhibits extremely low activities | Chaetomium erraticum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | for successful adsorption, enzyme to bentonite ratios greater than 0.4 (w/w) have to be used as lower ratios lead to 90% enzyme inactivation due to bentonite contact | Chaetomium erraticum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | KM-values of soluble and adsorbed enzymes do not differ significantly | Chaetomium erraticum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Chaetomium erraticum | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dextran + H2O | - |
Chaetomium erraticum | ? | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endo-dextranase | - |
Chaetomium erraticum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.4 | - |
soluble and adsorbed enzyme | Chaetomium erraticum |
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