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Literature summary for 3.2.1.11 extracted from
- Enzymic, spectroscopic and calorimetric studies of a recombinant dextranase expressed in Pichia pastoris (2003), Biotechnol. Appl. Biochem., 38, 211-221.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| crystallization of selenomethionyl-rDEX | Talaromyces minioluteus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 66000 | - |
1 * 66000 | Talaromyces minioluteus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dextran + H2O | Talaromyces minioluteus | - |
fragments of dextran | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Talaromyces minioluteus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | 10-12% of dextranase mass corresponds to carbohydrate in the form of N-linked oligosaccharide chains | Talaromyces minioluteus |
| additional information | rDEX has 2 disulphide bridges | Talaromyces minioluteus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 950 | 1000 | recombinant dextranase | Talaromyces minioluteus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dextran + H2O | - |
Talaromyces minioluteus | fragments of dextran | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 66000 | Talaromyces minioluteus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| rDex | recombinant dextranase | Talaromyces minioluteus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
50% loss of activity after 7.6 h | Talaromyces minioluteus |
| 60 | - |
thermal denaturation | Talaromyces minioluteus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 5 | - |
Talaromyces minioluteus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 6 | approx. 50% of maximal activity at pH 6.3, complete loss of activity above pH 7.5 | Talaromyces minioluteus |
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Release 2023.1 (January 2023)
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