Literature summary for 3.2.1.108 extracted from

Fabre, J.W.; Salehi, S.; Eckley, L.; Sawyer, G.J.; Zhang, X.; Dong, X.; Freund, J.N.
Intestinal lactase as an autologous beta-galactosidase reporter gene for in vivo gene expression studies (2008), Hum. Gene Ther., 20, 21-30.

Search for more literature for 3.2.1.108

Cloned(Commentary)

Cloned (Comment)	Organism
expression in HuH-7 cells	Escherichia coli
expression in HuH-7 cells	Rattus norvegicus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
brush border	full-length lactase form, mature lactase form with domain III and IV	Rattus norvegicus	5903	-
cytosol	-	Escherichia coli	5829	-
cytosol	water-soluble form of mature lactase with domain III and IV	Rattus norvegicus	5829	-
nucleus	-	Escherichia coli	5634	-
nucleus	water-soluble form of mature lactase with domain III and IV	Rattus norvegicus	5634	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
lactose + H2O	Rattus norvegicus	-	D-galactose + D-glucose	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-
Rattus norvegicus	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
small intestine epithelium	-	Rattus norvegicus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
5-bromo-4-chloro-3-indolyl-beta-D-fucopyranoside + H2O	37°C, 1 hour, full-length lactase, mature lactase, cytosolic form of mature lactase	Rattus norvegicus	?	-	?
5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside + H2O	37°C, 1 h	Escherichia coli	?	-	?
5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside + H2O	37°C, 1 hour, only cytosolic form of mature lactase	Rattus norvegicus	?	-	?
fluorescein-di-beta-D-galactopyranoside + H2O	only cytosolic form of mature lactase	Rattus norvegicus	?	-	?
lactose + H2O	-	Rattus norvegicus	D-galactose + D-glucose	-	?

Subunits

Subunits	Comment	Organism
homodimer	-	Escherichia coli
homodimer	-	Rattus norvegicus

Synonyms

Synonyms	Comment	Organism
intestinal lactase	-	Rattus norvegicus
lactase-phlorizin hydrolase	-	Rattus norvegicus
LPH	-	Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	-	Escherichia coli

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Release 2023.1 (January 2023)