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Literature summary for 3.2.1.108 extracted from

  • Skovbjerg, H.; Noren, O.; Sjöström, H.; Danielsen, E.M.; Enevoldsen, B.S.
    Further characterization of intestinal lactase/phlorizin hydrolase (1982), Biochim. Biophys. Acta, 707, 89-97.
    View publication on PubMed
Search for more literature for 3.2.1.108

Inhibitors

Inhibitors Comment Organism Structure
phlorizin
-
 Homo sapiens
phlorizin competitive inhibitor of lactose hydrolysis Sus scrofa
Tris competitive Homo sapiens
Tris competitive Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1
-
 lactose
-
 Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
160000
-
 2 * 160000, SDS-PAGE Homo sapiens
160000
-
 2 * 160000, SDS-PAGE Sus scrofa
320000
-
-
 Homo sapiens
320000
-
 gel filtration Sus scrofa

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
small intestine
-
 Homo sapiens
-
small intestine
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
18
-
-
 Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellobiose + H2O 25% of the activity with lactose Sus scrofa 2 beta-D-glucose
-
 ?
cellobiose + H2O 14% of the activity with lactose Homo sapiens 2 beta-D-glucose
-
 ?
cellotetraose + 3 H2O 14% of the activity with lactose Sus scrofa 4 beta-D-glucose
-
 ?
cellotetraose + 3 H2O 9% of the activity with lactose Homo sapiens 4 beta-D-glucose
-
 ?
cellotriose + H2O 14% of the activity with lactose Homo sapiens 3 D-glucose
-
 ?
cellotriose + H2O 9% of the activity with lactose Sus scrofa 3 D-glucose
-
 ?
cellulose + H2O 0.1% of the activity with lactose Homo sapiens beta-D-glucose
-
 ?
cellulose + H2O 0.1% of the activity with lactose Sus scrofa beta-D-glucose
-
 ?
lactose + H2O
-
 Homo sapiens D-glucose + D-galactose
-
 ?
lactose + H2O
-
 Sus scrofa D-glucose + D-galactose
-
 ?
phlorizin + H2O
-
 Homo sapiens phloretin + D-glucose
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 160000, SDS-PAGE Homo sapiens
dimer 2 * 160000, SDS-PAGE Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.6 6.2
-
 Sus scrofa

pH Range

pH Minimum pH Maximum Comment Organism
4 10 pH 4.0: about 60% of maximal activity, pH 10.0: about 50% of maximal activity Sus scrofa