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Literature summary for 3.2.1.106 extracted from

  • Frade-Perez, M.D.; Hernandez-Cervantes, A.; Flores-Carreon, A.; Mora-Montes, H.M.
    Biochemical characterization of Candida albicans alpha-glucosidase I heterologously expressed in Escherichia coli (2010), Antonie van Leeuwenhoek, 98, 291-298.
    View publication on PubMed
Search for more literature for 3.2.1.106

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Candida albicans

Inhibitors

Inhibitors Comment Organism Structure
1-deoxynojirimycin
-
 Candida albicans
australine 39% inhibition at 0.02 mM Candida albicans
bromoconduritol 22% inhibition at 0.02 mM Candida albicans
castanospermine
-
 Candida albicans
D-glucose
-
 Candida albicans
mannose
-
 Candida albicans
additional information 2-deoxyglucose, 3-deoxyglucose or 6-deoxyglucose do not have any effect on the enzyme activity; the enzyme is completely insensitive to diethyl pyrocarbonate Candida albicans
N-ethylmaleimide
-
 Candida albicans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.133
-
 4-methylumbelliferyl alpha-D-glucopyranoside in 10 mM sodium phosphate buffer pH 7.0 and at 37°C Candida albicans

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum
-
 Candida albicans 5783
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47800
-
 subunit, calculated from amino acid sequence Candida albicans
50000
-
 subunit, SDS-PAGE Candida albicans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Glc3Man9GlcNAc2 + H2O Candida albicans alpha-glucosidase I removes the outermost alpha1,2-glucose unit from the N-linked core Glc3Man9GlcNAc2 D-glucose + Glc2Man9GlcNAc2
-
 ?

Organism

Organism UniProt Comment Textmining
Candida albicans Q2V621
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE Bio-Gel A column chromatography and Sephadex G-25 gel filtration Candida albicans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl alpha-D-glucopyranoside + H2O not a common substrate for alpha-glucosidase I Candida albicans 4-methylumbelliferone + alpha-D-glucopyranose
-
 ?
Glc3Man9GlcNAc2 + H2O alpha-glucosidase I removes the outermost alpha1,2-glucose unit from the N-linked core Glc3Man9GlcNAc2 Candida albicans D-glucose + Glc2Man9GlcNAc2
-
 ?

Synonyms

Synonyms Comment Organism
alpha-glucosidase I
-
 Candida albicans
Cwh41p
-
 Candida albicans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7 recombinant enzyme, in 10 mM sodium phosphate buffer and at 37°C Candida albicans

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0027
-
 in 10 mM sodium phosphate buffer pH 7.0 and at 37°C Candida albicans castanospermine
0.0029
-
 in 10 mM sodium phosphate buffer pH 7.0 and at 37°C Candida albicans 1-deoxynojirimycin
2.2
-
 in 10 mM sodium phosphate buffer pH 7.0 and at 37°C Candida albicans N-ethylmaleimide
2.9
-
 in 10 mM sodium phosphate buffer pH 7.0 and at 37°C Candida albicans D-glucose
9.1
-
 in 10 mM sodium phosphate buffer pH 7.0 and at 37°C Candida albicans mannose

General Information

General Information Comment Organism
physiological function alpha-glucosidase I is required for normal cell wall composition, and virulence of Candida albicans Candida albicans