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Literature summary for 3.2.1.106 extracted from

  • Faridmoayer, A.; Scaman, C.H.
    Truncations and functional carboxylic acid residues of yeast processing alpha-glucosidase I (2007), Glycoconj. J., 24, 429-437.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
yeast strains transformed with pHVX2-His carrying truncated forms of CWH41. Mutated plasmids pE580A and pD584A transformed into Escherichia coli DH5-alpha and when transformed into Saccharomyces cerevisiae AH22 for expression Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
D584A Cwht1p mutant, expressed at significantly lower concentrations than the parental Cwht1p, expressed at a lower concentration than mutant E580A, is inactive Saccharomyces cerevisiae
E580A Cwht1p mutant, expressed at significantly lower concentrations than the parental Cwht1p, is inactive Saccharomyces cerevisiae
additional information truncated form Cwht1p (E35-F833), with deletion of the N-terminus and transmembrane domain expressed as a catalytically active fragment while truncated forms Cwht2p (R320-F833) and Cwht3p (M526-F833) not detectable Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
94000
-
x * 94000, truncated form Cwht1p purified as a soluble non-glycosylated protein, SDS-PAGE Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-
Saccharomyces cerevisiae AH22
-
-
-

Purification (Commentary)

Purification (Comment) Organism
truncated form Cwht1p purified as a soluble non-glycosylated protein to approximately 90% homogeneity by a combination of ammonium sulfate precipitation, anion-exchange chromatography and immobilized metal affinity chromatography Saccharomyces cerevisiae

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
140
-
Cwht1p Saccharomyces cerevisiae
3000
-
soluble alpha-glucosidase I Saccharomyces cerevisiae
3600
-
Cwht1p, purified 7.4fold Saccharomyces cerevisiae

Storage Stability

Storage Stability Organism
-80°C Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-D-Glc-(1-2)-alpha-D-Glc-(1-3)-alpha-D-Glc-O-(CH2)8COOCH3 residues E613 and D617 play a crucial role in maintaining enzyme activity Saccharomyces cerevisiae ?
-
?
alpha-D-Glc-(1-2)-alpha-D-Glc-(1-3)-alpha-D-Glc-O-(CH2)8COOCH3 residues E613 and D617 play a crucial role in maintaining enzyme activity Saccharomyces cerevisiae AH22 ?
-
?

Subunits

Subunits Comment Organism
? x * 94000, truncated form Cwht1p purified as a soluble non-glycosylated protein, SDS-PAGE Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
alpha-glucosidase I
-
Saccharomyces cerevisiae
Cwh41p
-
Saccharomyces cerevisiae
mannosyl-oligosaccharide glucosidase
-
Saccharomyces cerevisiae
processing alpha-glucosidase I
-
Saccharomyces cerevisiae