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Literature summary for 3.2.1.106 extracted from
- Crystallization and preliminary X-ray analysis of Escherichia coli K12 YgjK protein, a member of glycosyl hydrolase family 63 (2004), Acta Crystallogr. Sect. D, 60, 1284-1285.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpressed in Escherichia coli BL21(DE3) | Escherichia coli |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| 1.8 A, hanging-drop vapour-diffusion method, 20°C, 30% PEG 8000, 0.6 M magnesium chloride, 100 mM Tris-HCl, pH 7.5, flash-frozen in a stream of nitrogen gas at -173°C | Escherichia coli |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 85900 | - |
SDS-PAGE | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Glc3Man9GlcNAc2 + H2O | Escherichia coli | - |
? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
BL21(DE3) | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| HiPrep 16/10 Phenyl FF column, dialysis against 20 mM Tris-HCl pH 7.5, Q-Sepharose 16/10 anion exchange column | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Glc3Man9GlcNAc2 + H2O | - |
Escherichia coli | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| K12 YgjK protein | - |
Escherichia coli |
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