BRENDA - Enzyme Database
show all sequences of 3.2.1.106

Overexpression, purification, and partial characterization of Saccharomyces cerevisiae processing alpha glucosidase I

Dhanawansa, R.; Faridmoayer, A.; Van der Merwe, G.; Li, Y.X.; Scaman, C.H.; Glycobiology 12, 229-234 (2002) View publication on PubMed

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
diethyl dicarbonate
55% inhibition at 1 mM after 30 min
Saccharomyces cerevisiae
ethyl-3-(3-(dimethylamino)propyl)carbodiimide
90% inhibition at 50 mM after 60 min at pH 6.8, 4°C, preincubation with deoxynorjirmycin prevents
Saccharomyces cerevisiae
kojibiose
-
Saccharomyces cerevisiae
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
proteolytic cleavage of the transmembrane form between residues Ala24 and Thr25 results in a soluble form
Saccharomyces cerevisiae
16020
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
96510
-
calculated from amino acid sequence
Saccharomyces cerevisiae
98000
-
SDS-PAGE
Saccharomyces cerevisiae
Organism
Organism
UniProt
Commentary
Textmining
Saccharomyces cerevisiae
-
-
-
Saccharomyces cerevisiae AH22
-
-
-
Posttranslational Modification
Posttranslational Modification
Commentary
Organism
glycoprotein
4 potential N-glycosylation sites
Saccharomyces cerevisiae
Purification (Commentary)
Purification (Commentary)
Organism
soluble form
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
8550
-
-
Saccharomyces cerevisiae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
alpha-D-Glc-(1-2)-alpha-D-Glc-(1-3)-alpha-D-Glc-O(CH2)8-COOCH3 + H2O
synthetic substrate
655684
Saccharomyces cerevisiae
beta-D-glucose + alpha-D-Glc-(1-3)-alpha-D-Glc-O(CH2)8-COOCH3
-
-
-
?
alpha-D-Glc-(1-2)-alpha-D-Glc-(1-3)-alpha-D-Glc-O(CH2)8-COOCH3 + H2O
synthetic substrate
655684
Saccharomyces cerevisiae AH22
beta-D-glucose + alpha-D-Glc-(1-3)-alpha-D-Glc-O(CH2)8-COOCH3
-
-
-
?
Glc3Man9GlcNAc2 + H2O
3 glucose residues are required for catalysis
655684
Saccharomyces cerevisiae
D-glucose + Glc2Man9GlcNAc2
-
-
-
?
Glc3Man9GlcNAc2 + H2O
3 glucose residues are required for catalysis
655684
Saccharomyces cerevisiae AH22
D-glucose + Glc2Man9GlcNAc2
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
CWH41
-
Saccharomyces cerevisiae
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.8
-
kojibiose
pH 6.8, 37°C
Saccharomyces cerevisiae
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
diethyl dicarbonate
55% inhibition at 1 mM after 30 min
Saccharomyces cerevisiae
ethyl-3-(3-(dimethylamino)propyl)carbodiimide
90% inhibition at 50 mM after 60 min at pH 6.8, 4°C, preincubation with deoxynorjirmycin prevents
Saccharomyces cerevisiae
kojibiose
-
Saccharomyces cerevisiae
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.8
-
kojibiose
pH 6.8, 37°C
Saccharomyces cerevisiae
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
proteolytic cleavage of the transmembrane form between residues Ala24 and Thr25 results in a soluble form
Saccharomyces cerevisiae
16020
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
96510
-
calculated from amino acid sequence
Saccharomyces cerevisiae
98000
-
SDS-PAGE
Saccharomyces cerevisiae
Posttranslational Modification (protein specific)
Posttranslational Modification
Commentary
Organism
glycoprotein
4 potential N-glycosylation sites
Saccharomyces cerevisiae
Purification (Commentary) (protein specific)
Commentary
Organism
soluble form
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
8550
-
-
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
alpha-D-Glc-(1-2)-alpha-D-Glc-(1-3)-alpha-D-Glc-O(CH2)8-COOCH3 + H2O
synthetic substrate
655684
Saccharomyces cerevisiae
beta-D-glucose + alpha-D-Glc-(1-3)-alpha-D-Glc-O(CH2)8-COOCH3
-
-
-
?
alpha-D-Glc-(1-2)-alpha-D-Glc-(1-3)-alpha-D-Glc-O(CH2)8-COOCH3 + H2O
synthetic substrate
655684
Saccharomyces cerevisiae AH22
beta-D-glucose + alpha-D-Glc-(1-3)-alpha-D-Glc-O(CH2)8-COOCH3
-
-
-
?
Glc3Man9GlcNAc2 + H2O
3 glucose residues are required for catalysis
655684
Saccharomyces cerevisiae
D-glucose + Glc2Man9GlcNAc2
-
-
-
?
Glc3Man9GlcNAc2 + H2O
3 glucose residues are required for catalysis
655684
Saccharomyces cerevisiae AH22
D-glucose + Glc2Man9GlcNAc2
-
-
-
?
Other publictions for EC 3.2.1.106
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Protein Variants
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
kcat/KM [mM/s]
kcat/KM [mM/s] (protein specific)
751418
Miyazaki
Crystal structure and substra ...
Thermus thermophilus
J. Struct. Biol.
190
21-30
2015
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1
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732555
Sadat
Glycosylation, hypogammaglobul ...
Homo sapiens
N. Engl. J. Med.
370
1615-1625
2014
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3
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1
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1
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1
1
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732631
Wang
OsMOGS is required for N-glyca ...
Oryza sativa
Plant J.
78
632-645
2014
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2
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715139
Miyazaki
Heterologous expression and ch ...
Aspergillus brasiliensis, Aspergillus brasiliensis ATCC 9642
Glycoconj. J.
28
563-571
2011
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1
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2
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2
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1
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6
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2
1
1
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701754
Frade-Perez
Biochemical characterization o ...
Candida albicans
Antonie van Leeuwenhoek
98
291-298
2010
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1
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8
1
1
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2
1
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8
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1
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2
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1
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5
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1
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5
9
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1
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1
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1
1
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700317
Zhu
Glycoengineered Acid alpha-Glu ...
Homo sapiens
Mol. Ther.
17
954-963
2009
-
1
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3
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1
1
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8
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1
1
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1
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4
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701729
Chang
Novel imino sugar derivatives ...
Bovine viral diarrhea virus 1, Dengue virus, West Nile virus
Antimicrob. Agents Chemother.
53
1501-1508
2009
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21
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1
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10
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702920
Boulaflous
Cytosolic N-terminal arginine- ...
Arabidopsis thaliana
BMC Plant Biol.
9
144
2009
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1
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4
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703194
Rawlings
Synthesis and biological chara ...
Rattus norvegicus
ChemBioChem
10
1101-1105
2009
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3
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1
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2
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694198
McVie-Wylie
Biochemical and pharmacologica ...
Cricetulus griseus
Mol. Genet. Metab.
94
448-455
2008
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1
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1
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1
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1
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6
-
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1
3
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6
1
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1
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3
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1
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3
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2
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1
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1
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3
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699548
Kurakata
Structural insights into the s ...
Escherichia coli
J. Mol. Biol.
381
116-128
2008
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2
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1
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9
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9
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1
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1
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680052
Faridmoayer
Truncations and functional car ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae AH22
Glycoconj. J.
24
429-437
2007
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1
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3
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7
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681008
Ruddock
N-glycan processing in ER qual ...
Homo sapiens, Mus musculus
J. Cell Sci.
119
4373-4380
2006
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2
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2
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682320
Saint-Jore-Dupas
Plant N-glycan processing enzy ...
Arabidopsis thaliana
Plant Cell
18
3182-3200
2006
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1
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1
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2
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6
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656338
Hakamata
-
Recognition properties of proc ...
Rattus norvegicus
J. Carbohydr. Chem.
23
27-39
2004
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6
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657267
Faridmoayer
An improved purification proce ...
Saccharomyces cerevisiae
Protein Expr. Purif.
33
11-18
2004
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1
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8
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667038
Tonozuka
Crystallization and preliminar ...
Escherichia coli
Acta Crystallogr. Sect. D
60
1284-1285
2004
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1
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655684
Dhanawansa
Overexpression, purification, ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae AH22
Glycobiology
12
229-234
2002
-
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3
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1
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2
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11
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1
1
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4
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1
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1
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1
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1
1
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1
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4
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656351
Gillmor
Alpha-glucosidase I is require ...
Arabidopsis thaliana
J. Cell. Biol.
156
1003-1013
2002
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5
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1
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655326
Boisson
Arabidopsis glucosidase I muta ...
Arabidopsis thaliana
EMBO J.
20
1010-1019
2001
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1
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1
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7
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1
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170959
Kosuge
Effect of inhibitors of glycop ...
Mus musculus, Mus musculus C57BL/6
Biol. Pharm. Bull.
23
1-5
2000
-
-
-
-
-
-
3
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-
-
11
-
141
-
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1
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13
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3
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11
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1
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-
13
-
-
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-
-
-
-
170954
Zamarripa-Morales
Entamoeba histolytica: identif ...
Entamoeba histolytica, Entamoeba histolytica HM1:IMSS
Exp. Parasitol.
93
109-115
1999
2
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-
-
-
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6
1
1
1
-
2
-
10
-
-
-
-
-
1
-
-
10
-
-
1
-
-
-
1
-
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2
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-
-
-
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-
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6
-
1
1
1
-
2
-
-
-
-
-
1
-
-
10
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
170955
Herscovics
Importance of glycosidases in ...
Homo sapiens, Mammalia
Biochim. Biophys. Acta
1473
96-107
1999
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2
1
-
-
-
5
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7
2
1
19
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2
-
-
-
-
-
-
-
-
25
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
1
-
-
-
-
-
5
-
-
7
2
1
19
-
-
-
-
-
-
-
-
25
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
170956
Herscovics
Processing glycosidases of Sac ...
Saccharomyces cerevisiae, Mammalia
Biochim. Biophys. Acta
1426
275-285
1999
1
2
1
-
-
-
6
-
6
2
2
5
-
4
-
1
2
-
-
1
-
-
13
1
3
-
-
-
-
1
-
-
-
-
-
-
1
2
1
-
-
-
-
-
6
-
-
6
2
2
5
-
-
1
2
-
1
-
-
13
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
170957
Palcic
Processing alpha-glucosidase I ...
Bos taurus, Saccharomyces cerevisiae
Glycoconj. J.
16
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1999
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4
-
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1
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16
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2
4
-
2
2
-
28
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
1
-
-
16
-
-
-
2
-
2
2
-
28
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
170958
Rush
Topological studies on the enz ...
Rattus norvegicus, Sus scrofa
Glycobiology
8
1207-1213
1998
2
2
-
-
-
1
2
-
10
-
-
10
-
2
-
-
-
-
-
2
-
-
12
-
-
-
-
-
-
-
-
-
-
1
-
-
2
2
-
-
-
-
1
-
2
1
-
10
-
-
10
-
-
-
-
-
2
-
-
12
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
170966
Simons
Cell wall 1,6-beta-glucan synt ...
Saccharomyces cerevisiae
EMBO J.
17
396-405
1998
-
-
1
-
-
-
2
-
2
-
-
6
-
4
-
-
-
-
-
-
-
-
7
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
2
-
-
6
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
170967
Romero
The yeast CWH41 gene encodes g ...
Saccharomyces cerevisiae
Glycobiology
7
997-1004
1997
-
-
-
-
-
-
2
-
3
-
1
4
-
14
-
1
-
-
-
-
-
-
8
-
4
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
3
-
1
4
-
-
1
-
-
-
-
-
8
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
170965
Kalz-Fuller
Localization of the human gluc ...
Homo sapiens
Genomics
34
442-443
1996
-
-
1
-
-
-
-
-
3
-
1
6
-
3
-
1
-
-
-
1
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
1
6
-
-
1
-
-
1
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
170968
Jiang
CWH41 encodes a novel endoplas ...
Saccharomyces cerevisiae
J. Bacteriol.
178
1162-1171
1996
1
-
1
-
-
-
-
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4
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2
2
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1
-
1
-
-
-
-
-
-
3
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
4
-
2
2
-
-
1
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
170964
Kalz-Fuller
Cloning and expression of gluc ...
Homo sapiens, Sus scrofa
Eur. J. Biochem.
231
344-351
1995
-
-
1
-
-
-
6
-
12
2
6
14
-
10
-
4
1
-
-
5
-
-
24
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
8
-
-
12
2
6
14
-
-
4
1
-
5
-
-
24
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
170970
Shailubhai
Glucosidase I, a transmembrane ...
Rattus norvegicus
J. Biol. Chem.
266
16587-16593
1991
1
-
-
-
-
-
2
-
11
-
1
6
-
3
-
2
1
-
-
1
-
1
8
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
-
11
-
1
6
-
-
2
1
-
1
-
1
8
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
170971
Bause
Purification and characterizat ...
Bos taurus, Homo sapiens, Sus scrofa
Eur. J. Biochem.
183
661-669
1989
-
-
-
-
-
1
15
2
10
3
1
19
-
11
-
4
1
-
-
7
2
1
39
2
-
2
-
-
-
1
1
-
-
2
-
-
-
-
-
-
-
-
1
-
19
2
2
10
3
1
19
-
-
4
1
-
7
2
1
39
2
2
-
-
-
1
1
-
-
-
-
-
-
-
-
170969
Shailubhai
Purification and characterizat ...
Bos taurus, Cavia porcellus, Mus musculus, Rattus norvegicus
Biochem. J.
247
555-562
1987
5
-
-
-
-
1
20
-
6
3
2
21
-
13
-
-
1
-
-
11
1
3
31
1
-
2
-
1
-
1
1
-
-
-
-
-
5
-
-
-
-
-
1
-
23
-
-
6
3
2
21
-
-
-
1
-
11
1
3
31
1
2
-
1
-
1
1
-
-
-
-
-
-
-
-
170948
Bause E.; Erkens
-
Purification and characterizat ...
Bos taurus, Saccharomyces cerevisiae
FEBS Lett.
206
208-212
1986
-
-
-
-
-
-
10
-
2
2
2
6
-
2
-
2
1
-
-
1
2
-
16
2
-
2
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
10
-
-
2
2
2
6
-
-
2
1
-
1
2
-
16
2
2
-
-
-
2
1
-
-
-
-
-
-
-
-
170961
Szumilo
Purification and properties of ...
Vigna radiata
Arch. Biochem. Biophys.
247
261-271
1986
1
-
-
-
-
-
15
-
3
1
1
8
-
4
-
1
1
1
-
2
1
4
17
-
-
-
-
2
-
1
1
1
-
2
-
-
1
-
-
-
-
-
-
-
19
2
-
3
1
1
8
-
-
1
1
-
2
1
4
17
-
-
-
2
-
1
1
1
-
-
-
-
-
-
-
170972
Schweden
Characterization of calf liver ...
Bos taurus
Arch. Biochem. Biophys.
248
335-340
1986
-
-
-
-
-
-
12
1
2
1
4
6
-
1
-
1
1
-
-
1
-
-
23
2
-
1
-
-
-
2
1
-
-
8
-
-
-
-
-
-
-
-
-
-
17
8
1
2
1
4
6
-
-
1
1
-
1
-
-
23
2
1
-
-
-
2
1
-
-
-
-
-
-
-
-
170950
Hettkamp
Purification by affinity chrom ...
Bos taurus
Eur. J. Biochem.
142
85-90
1984
1
-
-
-
-
-
6
-
3
-
2
7
-
1
-
-
1
-
-
1
1
2
13
1
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
6
-
-
3
-
2
7
-
-
-
1
-
1
1
2
13
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
170962
Hettkamp
Inhibition by nojirimycin and ...
Bos taurus
Biosci. Rep.
2
899-906
1982
2
-
-
-
-
-
3
-
3
-
-
5
-
1
-
-
-
1
-
1
-
-
8
-
-
-
-
-
-
1
-
-
-
1
-
-
2
-
-
-
-
-
-
-
4
1
-
3
-
-
5
-
-
-
-
-
1
-
-
8
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
170949
Kilker
Partial purification from Sacc ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae X-2180
J. Biol. Chem.
256
5299-5303
1981
1
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-
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4
4
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2
1
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6
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4
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1
-
-
-
3
1
15
-
-
-
-
-
-
1
1
-
-
-
-
-
1
-
-
-
-
-
4
-
4
-
-
2
1
-
6
-
-
-
1
-
-
3
1
15
-
-
-
-
-
1
1
-
-
-
-
-
-
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136905
Grinna
Substrate specificities of rat ...
Rattus norvegicus
J. Biol. Chem.
255
2255-2258
1980
-
-
-
-
-
-
2
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4
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1
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-
-
-
-
1
-
-
22
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
3
-
-
4
-
-
-
-
-
1
-
-
22
-
1
-
-
-
-
-
-
-
-
-
-
-
-
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170953
Michael
Partial purification and chara ...
Bos taurus
Arch. Biochem. Biophys.
199
249-258
1980
-
-
-
-
-
-
-
-
1
-
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3
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1
-
-
1
-
-
1
1
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
3
-
-
-
1
-
1
1
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
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170960
Elting
Characterization of a glucosid ...
Gallus gallus
J. Biol. Chem.
255
2325-2331
1980
1
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-
-
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1
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4
1
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8
-
1
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1
-
-
1
1
-
16
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
-
-
-
-
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2
-
-
4
1
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8
-
-
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1
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1
1
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16
-
-
-
-
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2
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170952
Grinna
Glycoprotein biosynthesis. Rat ...
Rattus norvegicus
J. Biol. Chem.
254
8814-8818
1979
1
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5
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1
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1
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1
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8
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1
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
5
-
-
5
1
-
4
-
-
-
-
-
1
-
-
8
-
1
-
-
-
1
-
-
-
-
-
-
-
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-
170963
Liu
Arrangement of glucose residue ...
Talaromyces funiculosus, Rattus norvegicus, Talaromyces funiculosus QM474
J. Biol. Chem.
254
4554-4559
1979
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2
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1
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9
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3
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1
-
-
1
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-
15
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
9
-
-
-
1
-
1
-
-
15
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-