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Literature summary for 3.2.1.106 extracted from

  • Shailubhai, K.; Pukazhenthi, B.S.; Saxena, E.S.; Varma, G.M.; Vijay, I.K.
    Glucosidase I, a transmembrane endoplasmic reticular glycoprotein with a luminal catalytic domain (1991), J. Biol. Chem., 266, 16587-16593.
    View publication on PubMed
Search for more literature for 3.2.1.106

Activating Compound

Activating Compound Comment Organism Structure
Lubrol Px activation Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
N-ethylmaleimide inhibition when membranes are disrupted with Lubrol PX or saponin, inhibition abolished completely in the presence of dithiothreitol Rattus norvegicus
Trypsin up to 40% inhibition in the presence of Lubrol PX, without Lubrol PX: no inhibition Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum endoplasmic reticulum-resident glycoprotein Rattus norvegicus 5783
-
endoplasmic reticulum rough endoplasmic reticulum Rattus norvegicus 5783
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endoplasmic reticulum catalytic site/active centre located at lumenal side of endoplasmic reticulum Rattus norvegicus 5783
-
membrane membrane-bound Rattus norvegicus 16020
-
membrane transmembrane, endoplasmic reticular N-linked glycoprotein with distinct domains within its structure. It contains distinct membrane anchoring and luminally oriented catalytic domains, the enzyme has a cytoplasmically exposed tail Rattus norvegicus 16020
-
membrane membrane orientation Rattus norvegicus 16020
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microsome
-
 Rattus norvegicus
-
-
additional information subcellular localization Rattus norvegicus
-
-
additional information transmembrane, endoplasmic reticular N-linked glycoprotein with distinct domains within its structure. It contains distinct membrane anchoring and luminally oriented catalytic domains, the enzyme has a cytoplasmically exposed tail Rattus norvegicus
-
-
additional information membrane orientation Rattus norvegicus
-
-
additional information subcellular distribution and orientation Rattus norvegicus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
85000
-
 SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Glc3Man9GlcNAc2 + H2O Rattus norvegicus hydrolyses specifically terminal alpha1,2-linked glucose residue ?
-
 ?
Glc3Man9GlcNAc2 + H2O Rattus norvegicus specific involvement in the N-linked oligosaccharide-processing pathway ?
-
 ?
Glc3Man9GlcNAc2 + H2O Rattus norvegicus involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins ?
-
 ?
additional information Rattus norvegicus involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins ?
-
 ?
additional information Rattus norvegicus involved in processing of asparagine-linked oligosaccharides ?
-
 ?
additional information Rattus norvegicus critical role in overall biosynthesis and regulation of protein N-glycosylation ?
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein N-glycoprotein, N-glycosylation about 2 kDa Rattus norvegicus
glycoprotein with high-mannose oligosaccharides Rattus norvegicus

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
mammary gland mammary microsomes Rattus norvegicus
-

Storage Stability

Storage Stability Organism
-70°C, 200 mM potassium phosphate buffer, pH 6.8, 0.8% Lubrol PX Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Glc3Man9GlcNAc2 + H2O hydrolyses specifically terminal alpha1,2-linked glucose residue Rattus norvegicus D-glucose + Glc2Man9GlcNAc2
-
 ?
Glc3Man9GlcNAc2 + H2O hydrolyses specifically terminal alpha1,2-linked glucose residue Rattus norvegicus ?
-
 ?
Glc3Man9GlcNAc2 + H2O specific involvement in the N-linked oligosaccharide-processing pathway Rattus norvegicus ?
-
 ?
Glc3Man9GlcNAc2 + H2O involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins Rattus norvegicus ?
-
 ?
Glc3Man9GlcNAc2-peptide + H2O hydrolyses specifically terminal alpha1,2-linked glucose residue Rattus norvegicus D-glucose + Glc2Man9GlcNAc2-peptide
-
 ?
additional information involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins Rattus norvegicus ?
-
 ?
additional information involved in processing of asparagine-linked oligosaccharides Rattus norvegicus ?
-
 ?
additional information critical role in overall biosynthesis and regulation of protein N-glycosylation Rattus norvegicus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 85000, SDS-PAGE, reducing conditions Rattus norvegicus