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Literature summary for 3.2.1.106 extracted from

  • Szumilo, T.; Kaushal, G.P.; Elbein, A.D.
    Purification and properties of glucosidase I from mung bean seedlings (1986), Arch. Biochem. Biophys., 247, 261-271.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Triton X-100 0.2% Triton X-100: 4 to 5fold activation, glucosidase I releases in a soluble form by treating microsomal particles with 1.5% Triton X-100, not an absolute requirement for Triton X-100 Vigna radiata

Inhibitors

Inhibitors Comment Organism Structure
1-deoxynojirimycin strong inhibition Vigna radiata
2,5-Dihydroxymethyl-3,4-dihydroxypyrrolidine plant pyrrolidine alkaloid, DMDP, 0.04 mM: 50% inhibition Vigna radiata
castanospermine plant indolizidine alkaloid, strong inhibition Vigna radiata
chloride inhibitory above 0.3 M, 0.5 M: 30% inhibition Vigna radiata
Co2+ quite inhibitory Vigna radiata
Cu2+ quite inhibitory Vigna radiata
ethanolamine
-
Vigna radiata
glycerol 2% or higher: 25% or more inhibition Vigna radiata
kojibiose 0.5 mM: 50% inhibition; alpha-1,2-linked glucose disaccharide Vigna radiata
additional information no inhibition by alpha-1,3-, alpha-1,4-, and alpha-1,6-linked glucose disaccharides; no inhibition by EDTA; no inhibition by nigerose, maltose, isomaltose at 2 mM; no inhibition by swainsonine, deoxymannojirimycin or 1,4-dideoxy-1,4-imino-D-mannitol Vigna radiata
phosphate inhibitory above 0.3 M, 0.5 M: 30% inhibition Vigna radiata
sulfate 0.1-0.2 M: 15-20% inhibition, somewhate more inhibitory at higher concentrations Vigna radiata
tricine
-
Vigna radiata
Tris 20 mM: 50% inhibition Vigna radiata
Zn2+ quite inhibitory Vigna radiata

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum
-
Vigna radiata 5783
-
membrane membrane-bound Vigna radiata 16020
-
microsome
-
Vigna radiata
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no requirement for divalent cations Vigna radiata

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
316000
-
gel fitration Vigna radiata

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Glc3Man9GlcNAc2 + H2O Vigna radiata hydrolyses specifically terminal alpha1,2-linked glucose residue ?
-
?
Glc3Man9GlcNAc2 + H2O Vigna radiata specific involvement in the N-linked oligosaccharide-processing pathway ?
-
?
Glc3Man9GlcNAc2 + H2O Vigna radiata involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins ?
-
?
Glc3Man9GlcNAc2 + H2O Vigna radiata involved in processing of asparagine-linked oligosaccharides ?
-
?
additional information Vigna radiata involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins ?
-
?
additional information Vigna radiata involved in processing of asparagine-linked oligosaccharides ?
-
?
additional information Vigna radiata first enzyme of processing of N-linked oligosaccharides in plants, plant glucosidase I might be analogous to glucosidase I of animal cells ?
-
?
additional information Vigna radiata involved in the formation of high mannose and complex glycoproteins ?
-
?

Organism

Organism UniProt Comment Textmining
Vigna radiata
-
mung bean
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein with high-mannose oligosaccharides Vigna radiata

Purification (Commentary)

Purification (Comment) Organism
-
Vigna radiata

Reaction

Reaction Comment Organism Reaction ID
Glc3Man9GlcNAc2-[protein] + H2O = Glc2Man9GlcNAc2-[protein] + beta-D-glucopyranose glucosidase I is a specific alpha-(1,2)-glucosidase Vigna radiata

Source Tissue

Source Tissue Comment Organism Textmining
seedling
-
Vigna radiata
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Vigna radiata

Storage Stability

Storage Stability Organism
-18°C, 10% glycerol, few weeks, no loss of activity Vigna radiata
0°C, very slight loss of activity Vigna radiata
27°C, 40 h, more than 50% activity retained Vigna radiata
37°C, most of activity lost during storage Vigna radiata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Glc3Man9GlcNAc + H2O hydrolyses specifically terminal alpha1,2-linked glucose residue Vigna radiata D-glucose + Glc2Man9GlcNAc
-
?
Glc3Man9GlcNAc2 + H2O hydrolyses specifically terminal alpha1,2-linked glucose residue Vigna radiata D-glucose + Glc2Man9GlcNAc2
-
?
Glc3Man9GlcNAc2 + H2O hydrolyses specifically terminal alpha1,2-linked glucose residue Vigna radiata ?
-
?
Glc3Man9GlcNAc2 + H2O specific involvement in the N-linked oligosaccharide-processing pathway Vigna radiata ?
-
?
Glc3Man9GlcNAc2 + H2O involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins Vigna radiata ?
-
?
Glc3Man9GlcNAc2 + H2O involved in processing of asparagine-linked oligosaccharides Vigna radiata ?
-
?
Glc3Man9GlcNAc2-lipid + H2O hydrolyses specifically terminal alpha1,2-linked glucose residue Vigna radiata D-glucose + Glc2Man9GlcNAc2-lipid
-
?
Glc3Man9GlcNAc2-peptide + H2O hydrolyses specifically terminal alpha1,2-linked glucose residue Vigna radiata D-glucose + Glc2Man9GlcNAc2-peptide
-
?
additional information Glc1Man9GlcNAc2: not a substrate Vigna radiata ?
-
?
additional information also acts, more slowly, on the corresponding glycolipids and glycopeptides Vigna radiata ?
-
?
additional information p-nitrophenyl-alpha-D-glucopyranoside: not a substrate Vigna radiata ?
-
?
additional information Glc2Man9GlcNAc2: not a substrate Vigna radiata ?
-
?
additional information substrate and product specificity Vigna radiata ?
-
?
additional information involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins Vigna radiata ?
-
?
additional information involved in processing of asparagine-linked oligosaccharides Vigna radiata ?
-
?
additional information first enzyme of processing of N-linked oligosaccharides in plants, plant glucosidase I might be analogous to glucosidase I of animal cells Vigna radiata ?
-
?
additional information involved in the formation of high mannose and complex glycoproteins Vigna radiata ?
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
27
-
40 h: 50% activity retained Vigna radiata
37
-
most activity lost during storage Vigna radiata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3 6.5
-
Vigna radiata

pH Range

pH Minimum pH Maximum Comment Organism
5.5 6.8 activity greatly decreases at pH values below 5.5 or above 6.8 Vigna radiata

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5 7 most stable at Vigna radiata

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.04
-
2,5-Dihydroxymethyl-3,4-dihydroxypyrrolidine plant pyrrolidine alkaloid, DMDP Vigna radiata
0.5
-
kojibiose
-
Vigna radiata