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Literature summary for 3.2.1.106 extracted from

  • Elting, J.J.; Chen, W.W.; Lennarz, W.J.
    Characterization of a glucosidase involved in an initial step in the processing of oligosaccharide chains (1980), J. Biol. Chem., 255, 2325-2331.
    View publication on PubMed
Search for more literature for 3.2.1.106

Activating Compound

Activating Compound Comment Organism Structure
NP-40 crude enzyme: 3 to 10fold activation Gallus gallus

Inhibitors

Inhibitors Comment Organism Structure
additional information no inhibition by alpha- or beta-linked glucose disaccharides; no inhibition by methyl-beta-D-glucoside, sophorose laminaribiose, cellobiose, gentiobiose and methyl-alpha-D-glucoside Gallus gallus

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum rough endoplasmic reticulum Gallus gallus 5783
-
membrane membrane-bound Gallus gallus 16020
-
microsome
-
 Gallus gallus
-
-
additional information subcellular localization Gallus gallus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
phosphate activation Gallus gallus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Glc3Man9GlcNAc2 + H2O Gallus gallus hydrolyses specifically terminal alpha1,2-linked glucose residue ?
-
 ?
Glc3Man9GlcNAc2 + H2O Gallus gallus catalyzes initial step in post-glycosylation processing of cellular glycoprotein ?
-
 ?
Glc3Man9GlcNAc2 + H2O Gallus gallus involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins ?
-
 ?
Glc3Man9GlcNAc2 + H2O Gallus gallus involved in processing of asparagine-linked oligosaccharides ?
-
 ?
additional information Gallus gallus catalyzes initial step in post-glycosylation processing of cellular glycoprotein ?
-
 ?
additional information Gallus gallus involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins ?
-
 ?
additional information Gallus gallus involved in processing of asparagine-linked oligosaccharides ?
-
 ?
additional information Gallus gallus involved in the formation of high mannose and complex glycoproteins ?
-
 ?

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
 hen
-

Purification (Commentary)

Purification (Comment) Organism
-
 Gallus gallus

Source Tissue

Source Tissue Comment Organism Textmining
oviduct
-
 Gallus gallus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Gallus gallus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Glc3Man9GlcNAc2 + H2O hydrolyses specifically terminal alpha1,2-linked glucose residue Gallus gallus D-glucose + Glc2Man9GlcNAc2
-
 ?
Glc3Man9GlcNAc2 + H2O hydrolyses specifically terminal alpha1,2-linked glucose residue Gallus gallus ?
-
 ?
Glc3Man9GlcNAc2 + H2O catalyzes initial step in post-glycosylation processing of cellular glycoprotein Gallus gallus ?
-
 ?
Glc3Man9GlcNAc2 + H2O involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins Gallus gallus ?
-
 ?
Glc3Man9GlcNAc2 + H2O involved in processing of asparagine-linked oligosaccharides Gallus gallus ?
-
 ?
Glc3Man9GlcNAc2-lipid + H2O hydrolyses specifically terminal alpha1,2-linked glucose residue Gallus gallus D-glucose + Glc2Man9GlcNAc2-lipid
-
 ?
Glc3Man9GlcNAc2-lipid + H2O activity with oligosaccharide-lipid 2.5 times higher than with glycosylated alpha-lactalbumin and 3 times higher than with free oligosaccharide Gallus gallus D-glucose + Glc2Man9GlcNAc2-lipid
-
 ?
Glc3Man9GlcNAc2-peptide + H2O hydrolyses specifically terminal alpha1,2-linked glucose residue Gallus gallus D-glucose + Glc2Man9GlcNAc2-peptide
-
 ?
Glc3Man9GlcNAc2-peptide + H2O Glc-containing oligosaccharide attached to S-carboxymethylated alpha-lactalbumin Gallus gallus D-glucose + Glc2Man9GlcNAc2-peptide
-
 ?
additional information also acts, more slowly, on the corresponding glycolipids and glycopeptides Gallus gallus ?
-
 ?
additional information substrate selectivity Gallus gallus ?
-
 ?
additional information p-NO2-phenyl-alpha-glucoside and p-NO2-phenyl-beta-glucoside: no substrates Gallus gallus ?
-
 ?
additional information catalyzes initial step in post-glycosylation processing of cellular glycoprotein Gallus gallus ?
-
 ?
additional information involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins Gallus gallus ?
-
 ?
additional information involved in processing of asparagine-linked oligosaccharides Gallus gallus ?
-
 ?
additional information involved in the formation of high mannose and complex glycoproteins Gallus gallus ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
 near neutral pH optimum Gallus gallus
6.5 8 broad pH optimum between Gallus gallus