Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Triton X-100 | about 45% activation of membrane-bound activity, maximum stimulation of membrane-bound activity at 0.5% Triton X-100, no activation of soluble activity | Saccharomyces cerevisiae |
Application | Comment | Organism |
---|---|---|
analysis | contributes to knowledge of N-glycan biosynthesis | Saccharomyces cerevisiae |
pharmacology | glucosidase I may be a target for antiviral and antitumor drugs, glucosidase I inhibitors are potentially useful as antiviral, antitumor and antimetastatic agents | Mammalia |
Cloned (Comment) | Organism |
---|---|
CWH41 gene, encodes yeast glucosidase I | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-deoxynojirimycin | strong inhibition | Mammalia | |
1-deoxynojirimycin | strong inhibition | Saccharomyces cerevisiae | |
castanospermine | - |
Mammalia | |
castanospermine | - |
Saccharomyces cerevisiae | |
kojibiose | - |
Saccharomyces cerevisiae | |
Tris | - |
Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | endoplasmic reticulum-resident glycoprotein | Mammalia | 5783 | - |
endoplasmic reticulum | endoplasmic reticulum-resident glycoprotein | Saccharomyces cerevisiae | 5783 | - |
membrane | type-II transmembrane protein | Mammalia | 16020 | - |
membrane | type-II transmembrane protein | Saccharomyces cerevisiae | 16020 | - |
additional information | X-2180, equally distributed between particulate and supernatant fractions | Saccharomyces cerevisiae | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no requirement for divalent cations | Mammalia | |
additional information | no requirement for divalent cations | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
95000 | - |
proteolytically generated soluble form of the enzyme, SDS-PAGE | Saccharomyces cerevisiae |
107000 | - |
cloned CWH41 gene encodes a type-II transmembrane protein, Cwh41p, of 107 kDa, SDS-PAGE | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Glc3Man9GlcNAc2 + H2O | Mammalia | hydrolyses specifically terminal alpha1,2-linked glucose residue | ? | - |
? | |
Glc3Man9GlcNAc2 + H2O | Saccharomyces cerevisiae | hydrolyses specifically terminal alpha1,2-linked glucose residue | ? | - |
? | |
additional information | Saccharomyces cerevisiae | indirect role in cell wall beta1,6-glucan biosynthesis and/or in beta1,6-glucan insertion into the cell wall | ? | - |
? | |
additional information | Mammalia | trimming of the glucose residues is identical in Saccharomyces cerevisiae and in mammalian cells | ? | - |
? | |
additional information | Saccharomyces cerevisiae | trimming of the glucose residues is identical in Saccharomyces cerevisiae and in mammalian cells | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mammalia | - |
- |
- |
Saccharomyces cerevisiae | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Saccharomyces cerevisiae |
Purification (Comment) | Organism |
---|---|
partial | Saccharomyces cerevisiae |
partial, soluble glucosidase I | Saccharomyces cerevisiae |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-D-Glc-(1-2)-alpha-D-Glc-(1-3)-alpha-D-Glc-O(CH2)8-COOCH3 + H2O | synthetic trisaccharide | Saccharomyces cerevisiae | beta-D-glucose + alpha-D-Glc-(1-3)-alpha-D-Glc-O(CH2)8-COOCH3 | - |
? | |
Glc3Man9GlcNAc2 + H2O | hydrolyses specifically terminal alpha1,2-linked glucose residue | Mammalia | D-glucose + Glc2Man9GlcNAc2 | - |
? | |
Glc3Man9GlcNAc2 + H2O | hydrolyses specifically terminal alpha1,2-linked glucose residue | Saccharomyces cerevisiae | D-glucose + Glc2Man9GlcNAc2 | - |
? | |
Glc3Man9GlcNAc2 + H2O | hydrolyses specifically terminal alpha1,2-linked glucose residue | Mammalia | ? | - |
? | |
Glc3Man9GlcNAc2 + H2O | hydrolyses specifically terminal alpha1,2-linked glucose residue | Saccharomyces cerevisiae | ? | - |
? | |
additional information | Glc1Man9GlcNAc2: not a substrate | Saccharomyces cerevisiae | ? | - |
? | |
additional information | also acts, more slowly, on the corresponding glycolipids and glycopeptides | Mammalia | ? | - |
? | |
additional information | also acts, more slowly, on the corresponding glycolipids and glycopeptides | Saccharomyces cerevisiae | ? | - |
? | |
additional information | p-nitrophenyl-alpha-D-glucopyranoside: not a substrate | Saccharomyces cerevisiae | ? | - |
? | |
additional information | Glc2Man9GlcNAc2: not a substrate | Saccharomyces cerevisiae | ? | - |
? | |
additional information | indirect role in cell wall beta1,6-glucan biosynthesis and/or in beta1,6-glucan insertion into the cell wall | Saccharomyces cerevisiae | ? | - |
? | |
additional information | trimming of the glucose residues is identical in Saccharomyces cerevisiae and in mammalian cells | Mammalia | ? | - |
? | |
additional information | trimming of the glucose residues is identical in Saccharomyces cerevisiae and in mammalian cells | Saccharomyces cerevisiae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 95000, SDS-PAGE, reducing conditions | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
CWH41 | - |
Saccharomyces cerevisiae |
Cwh41p | calcofluor white hypersensitivity | Saccharomyces cerevisiae |
Cwh41p | CWH41 gene encodes a type-II transmembrane protein, Cwh41p | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
- |
Saccharomyces cerevisiae |