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Literature summary for 3.2.1.106 extracted from

  • Grinna, L.S.; Robbins, P.W.
    Glycoprotein biosynthesis. Rat liver microsomal glucosidases which process oligosaccharides (1979), J. Biol. Chem., 254, 8814-8818.
    View publication on PubMed
Search for more literature for 3.2.1.106

Activating Compound

Activating Compound Comment Organism Structure
Triton X-100 activation, required in order to measure glucosidase activity Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
D-glucose 5 mM: 25% inhibition Rattus norvegicus
D-mannose 5 mM: 25% inhibition Rattus norvegicus
additional information no inhibition by EDTA Rattus norvegicus
p-nitrophenyl-alpha-D-glucoside 5 mM Rattus norvegicus
p-nitrophenyl-beta-D-glucoside 5 mM Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum rough and smooth endoplasmic reticulum Rattus norvegicus 5783
-
membrane integral membrane protein Rattus norvegicus 16020
-
membrane microsomal membranes Rattus norvegicus 16020
-
microsome localized in rough and smooth microsomes, located on the cisternal surface of microsomal vesicles Rattus norvegicus
-
-
additional information subcellular distribution and orientation Rattus norvegicus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no requirement for divalent cations Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Glc3Man9GlcNAc2 + H2O Rattus norvegicus catalyzes initial step in post-glycosylation processing of cellular glycoprotein ?
-
 ?
Glc3Man9GlcNAc2 + H2O Rattus norvegicus involved in processing of asparagine-linked oligosaccharides ?
-
 ?
additional information Rattus norvegicus catalyzes initial step in post-glycosylation processing of cellular glycoprotein ?
-
 ?
additional information Rattus norvegicus involved in processing of asparagine-linked oligosaccharides ?
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Reaction

Reaction Comment Organism Reaction ID
Glc3Man9GlcNAc2-[protein] + H2O = Glc2Man9GlcNAc2-[protein] + beta-D-glucopyranose glucosidase I is a specific alpha-(1,2)-glucosidase Rattus norvegicus
a

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Glc3Man9GlcNAc + H2O hydrolyses specifically terminal alpha1,2-linked glucose residue Rattus norvegicus D-glucose + Glc2Man9GlcNAc
-
 ?
Glc3Man9GlcNAc2 + H2O
-
 Rattus norvegicus D-glucose + Glc2Man9GlcNAc2
-
 ?
Glc3Man9GlcNAc2 + H2O catalyzes initial step in post-glycosylation processing of cellular glycoprotein Rattus norvegicus ?
-
 ?
Glc3Man9GlcNAc2 + H2O involved in processing of asparagine-linked oligosaccharides Rattus norvegicus ?
-
 ?
Glc3Man9GlcNAc2-lipid + H2O hydrolyses specifically terminal alpha1,2-linked glucose residue Rattus norvegicus D-glucose + Glc2Man9GlcNAc2-lipid
-
 ?
Glc3Man9GlcNAc2-peptide + H2O hydrolyses specifically terminal alpha1,2-linked glucose residue Rattus norvegicus D-glucose + Glc2Man9GlcNAc2-peptide
-
 ?
additional information catalyzes initial step in post-glycosylation processing of cellular glycoprotein Rattus norvegicus ?
-
 ?
additional information involved in processing of asparagine-linked oligosaccharides Rattus norvegicus ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7.5 broad pH optimum between Rattus norvegicus