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Literature summary for 3.2.1.104 extracted from

  • Watanabe, T.; Ito, T.; Goda, H.; Ishibashi, Y.; Miyamoto, T.; Ikeda, K.; Taguchi, R.; Okino, N.; Ito, M.
    Sterylglucoside catabolism in cryptococcus neoformans with endoglycoceramidase-related protein 2 (EGCrP2), the first steryl-beta-glucosidase identified in fungi (2015), J. Biol. Chem., 290, 1005-1019 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.104

Application

Application Comment Organism
pharmacology the enzyme may be a promising target for anti-cryptococcal drugs Cryptococcus neoformans

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) Cryptococcus neoformans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.034
-
 4-methylumbelliferyl beta-D-glucoside pH 6.0, 30°C Cryptococcus neoformans
0.0384
-
 (6-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]hexanoyl)-beta-D-glucosylceramide pH 6.0, 30°C Cryptococcus neoformans
0.0436
-
 resorufin 3-O-beta-D-glucoside pH 6.0, 30°C Cryptococcus neoformans
0.817
-
 4-nitrophenyl beta-D-glucoside pH 6.0, 30°C Cryptococcus neoformans

Localization

Localization Comment Organism GeneOntology No. Textmining
vacuole
-
 Cryptococcus neoformans 5773
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
120000
-
 SDS-PAGE Cryptococcus neoformans

Organism

Organism UniProt Comment Textmining
Cryptococcus neoformans A0A228BX09
-
-
Cryptococcus neoformans ATCC 208821 A0A228BX09
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Cryptococcus neoformans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(6-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]hexanoyl)-beta-D-glucosylceramide + H2O
-
 Cryptococcus neoformans D-glucose + 6-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]hexanol
-
 ?
(6-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]hexanoyl)-beta-D-glucosylceramide + H2O
-
 Cryptococcus neoformans ATCC 208821 D-glucose + 6-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]hexanol
-
 ?
4-methylumbelliferyl beta-D-glucoside + H2O
-
 Cryptococcus neoformans D-glucose + 4-methylumbelliferone
-
 ?
4-methylumbelliferyl beta-D-glucoside + H2O
-
 Cryptococcus neoformans ATCC 208821 D-glucose + 4-methylumbelliferone
-
 ?
4-nitrophenyl beta-D-glucoside + H2O
-
 Cryptococcus neoformans D-glucose + 4-nitrophenol
-
 ?
4-nitrophenyl beta-D-glucoside + H2O
-
 Cryptococcus neoformans ATCC 208821 D-glucose + 4-nitrophenol
-
 ?
arbutin + H2O
-
 Cryptococcus neoformans D-glucose + benzene-1,4-diol
-
 ?
cholesteryl 3-O-beta-glucoside + H2O
-
 Cryptococcus neoformans D-glucose + cholesterol
-
 ?
ergosteryl 3-O-beta-glucoside + H2O
-
 Cryptococcus neoformans D-glucose + erosterol
-
 ?
glucosylceramide + H2O
-
 Cryptococcus neoformans D-glucose + ceramide
-
 ?
glucosylceramide + H2O
-
 Cryptococcus neoformans ATCC 208821 D-glucose + ceramide
-
 ?
indoxyl beta-D-glucoside + H2O
-
 Cryptococcus neoformans D-glucose + indoxyl
-
 ?
additional information no activity with alpha-glucosides or beta-galactosides, under acidic conditions Cryptococcus neoformans ?
-
 ?
additional information no activity with alpha-glucosides or beta-galactosides, under acidic conditions Cryptococcus neoformans ATCC 208821 ?
-
 ?
n-octyl beta-D-glucoside + H2O
-
 Cryptococcus neoformans D-glucose + n-octanol
-
 ?
phloridzin + H2O
-
 Cryptococcus neoformans D-glucose + phloretin
-
 ?
resorufin 3-O-beta-D-glucoside + H2O
-
 Cryptococcus neoformans D-glucose + resorufin
-
 ?
salicin + H2O
-
 Cryptococcus neoformans D-glucose + 2-(hydroxymethyl)phenol
-
 ?
sitosteryl 3-O-beta-glucoside + H2O
-
 Cryptococcus neoformans D-glucose + sitosterol
-
 ?

Subunits

Subunits Comment Organism
? x * 120000, SDS-PAGE Cryptococcus neoformans

Synonyms

Synonyms Comment Organism
EGCrP2
-
 Cryptococcus neoformans
endoglycoceramidase-related protein 2
-
 Cryptococcus neoformans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Cryptococcus neoformans
32 37
-
 Cryptococcus neoformans

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
16 40 16°C: about 50% of maximal activity, 40°C: about 75% of maximal activity Cryptococcus neoformans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.53
-
 (6-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]hexanoyl)-beta-D-glucosylceramide pH 6.0, 30°C Cryptococcus neoformans
8.8
-
 4-methylumbelliferyl beta-D-glucoside pH 6.0, 30°C Cryptococcus neoformans
13.8
-
 4-nitrophenyl beta-D-glucoside pH 6.0, 30°C Cryptococcus neoformans
21.9
-
 resorufin 3-O-beta-D-glucoside pH 6.0, 30°C Cryptococcus neoformans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 5.5
-
 Cryptococcus neoformans
6
-
 assay at Cryptococcus neoformans

pH Range

pH Minimum pH Maximum Comment Organism
4 7 pH 4.0: about % of maximal activity, pH 7.0: about % of maximal activity Cryptococcus neoformans

General Information

General Information Comment Organism
malfunction disruption of the EGCrP2 gene (egcrp2) results in the accumulation of ergosteryl 3-O-beta-glucoside, a major sterylglucoside in fungi. Distinct growth arrest, a dysfunction in cell budding, and an abnormal vacuole morphology are detected in the egcrp2-disrupted mutants Cryptococcus neoformans
physiological function the enzyme is involved in the catabolism of ergosteryl 3-O-beta-glucoside in the vacuoles of Cryptococcus neoformans Cryptococcus neoformans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
13.8
-
 (6-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]hexanoyl)-beta-D-glucosylceramide pH 6.0, 30°C Cryptococcus neoformans
16.9
-
 4-nitrophenyl beta-D-glucoside pH 6.0, 30°C Cryptococcus neoformans
25.7
-
 4-methylumbelliferyl beta-D-glucoside pH 6.0, 30°C Cryptococcus neoformans
504
-
 resorufin 3-O-beta-D-glucoside pH 6.0, 30°C Cryptococcus neoformans