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Literature summary for 3.2.1.103 extracted from

  • Scudder, P.; Uemura, K.i.; Dolby, J.; Fukuda, M.N.; Feizi, T.
    Isolation and characterization of an endo-beta-galactosidase from Bacteroides fragilis (1983), Biochem. J., 213, 484-494.
No PubMed abstract available
Search for more literature for 3.2.1.103

General Stability

General Stability Organism
Ca2+ stabilizes Bacteroides fragilis

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 1 mM, partial inhibition Bacteroides fragilis
Fe2+ 1 mM, partial inhibition Bacteroides fragilis
Galbeta1-4GlcNAcbeta1-3Gal when assayed with lacto-N-neotetraosylceramide Bacteroides fragilis
GlcNAcbeta1-3Gal when assayed with lacto-N-neotetraosylceramide Bacteroides fragilis
PCMB 1 mM, complete inhibition Bacteroides fragilis
Zn2+ 1 mM, partial inhibition Bacteroides fragilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 gel filtration Bacteroides fragilis

Organism

Organism UniProt Comment Textmining
Bacteroides fragilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacteroides fragilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
156
-
-
 Bacteroides fragilis

Storage Stability

Storage Stability Organism
-20°C, 0.044 mg/ml protein, stable for at least 12 months Bacteroides fragilis
4°C, stable for at least 2-3 months at a protein concentration of 0.044 mg/ml protein, 20% loss of activity in 3 h at a protein concentration of 0.003 mg/ml Bacteroides fragilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Galbeta1-3GlcNAcbeta1-3Galbeta1-4Glc + H2O
-
 Bacteroides fragilis ?
-
 ?
Galbeta1-3[Fucalpha1-4]GlcNAcbeta1-3Galbeta1-4Glc + H2O
-
 Bacteroides fragilis ?
-
 ?
Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glc + H2O
-
 Bacteroides fragilis ?
-
 ?
keratan sulfate + H2O from bovine cornea Bacteroides fragilis keratan oligosaccharides
-
 ?
lacto-N-neotetraosylceramide + H2O
-
 Bacteroides fragilis ?
-
 ?
additional information hydrolyzes the type 2 sequence Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glc at about twice the rate of the type 1 isomer, Galbeta1-3GlcNAcbeta1-3Galbeta1-4Glc Bacteroides fragilis ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Bacteroides fragilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 0.003 mg/ml protein, 40% loss of activity after 30 min Bacteroides fragilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.7
-
-
 Bacteroides fragilis

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.5 9 25°C, 16 h, stable in presence of 0.2 mg of albumin per ml Bacteroides fragilis