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Literature summary for 3.2.1.103 extracted from
- endo-beta-Galactosidase from Flavobacterium keratolyticus (1982), Methods Enzymol., 83, 619-625.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | - |
Sphingobacterium multivorum |  |
| Cu2+ | - |
Sphingobacterium multivorum | |
| Hg2+ | - |
Sphingobacterium multivorum | |
| PCMB | - |
Sphingobacterium multivorum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
gel filtration | Sphingobacterium multivorum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Sphingobacterium multivorum | the enzyme is produced without induction by keratan sulfate | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sphingobacterium multivorum | - |
- |
- |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 20°C, stable for at least 5 months | Sphingobacterium multivorum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| erythroglycan + H2O | - |
Sphingobacterium multivorum | ? | - |
? | |
| Fucalpha1-2Galbeta1-3GlcNAcbeta1-3Galbeta1-4Glc + H2O | - |
Sphingobacterium multivorum | ? | - |
? | |
| Galbeta1-3GlcNAc(6-2alphaNeuAc)beta1-3Galbeta1-4Glc + H2O | - |
Sphingobacterium multivorum | ? | - |
? | |
| Galbeta1-3GlcNAcbeta1-3Galbeta1-4Glc + H2O | - |
Sphingobacterium multivorum | ? | - |
? | |
| Galbeta1-3[Fucalpha1-4]GlcNAcbeta1-3Galbeta1-4Glc + H2O | - |
Sphingobacterium multivorum | ? | - |
? | |
| Galbeta1-4GlcNAcbeta1-3Galbeta1-3Galbeta1-4Glc-Cer + H2O | - |
Sphingobacterium multivorum | Galbeta1-4GlcNAcbeta1-3Galbeta1-3Gal + Glc-Cer | - |
? | |
| Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glc + H2O | - |
Sphingobacterium multivorum | ? | - |
? | |
| keratan sulfate + H2O | from nasal carilage | Sphingobacterium multivorum | keratan oligosaccharides | - |
? | |
| additional information | hydrolyzes glycoconjugates containing N-acetyllactosamine repeating units | Sphingobacterium multivorum | ? | - |
? | |
| additional information | the enzyme is produced without induction by keratan sulfate | Sphingobacterium multivorum | ? | - |
? | |
| NeuAcalpha2-3Galbeta1-3GlcNAc(6-2alphaNeuAc)beta1-3Galbeta1-4Glc + H2O | - |
Sphingobacterium multivorum | ? | - |
? | |
| NeuAcalpha2-3Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glc-Cer + H2O | - |
Sphingobacterium multivorum | NeuAcalpha2-3Galbeta1-4GlcNAcbeta1-3Gal + Glc-Cer | - |
? | |
| NeuAcalpha2-6Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glc + H2O | - |
Sphingobacterium multivorum | ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
hydrolysis of keratan sulfate | Sphingobacterium multivorum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
rapid loss of activity below | Sphingobacterium multivorum |
| 5 | 10 | stable | Sphingobacterium multivorum |
| 11 | - |
rapid loss of activity above | Sphingobacterium multivorum |
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